Copper nitrate trihydrate (Cu(NO3)2⋅3H2O), sodium hydroxide (NaOH), sodium chloride (NaCl), potassium ferricyanide, potassium ferrocyanide, glucose, ethanol, AA, UA, and AP are analytical pure and were bought from Sinopharm Chemical Reagent Co., Ltd. BSA was purchased from Aladdin with purity of 96%. Nafion 117 solution is analytical purity, produced by Sigma-Aldrich Company, USA. All of reagents were used without further purification.
The crystalline structure of Cu2O–BSA NPs was detected by an X-ray diffractometer (Bruker D8 ADVANCE) with Cu-Kα radiation (λ = 1.5406 Å). A type of H-7650 TEM (Japan) was used to observe the size and morphology of the Cu2O–BSA NPs. A CHI 690 electrochemical workstation (Chenhua, Shanghai, China) was assumed to conduct the electrochemical experiments including CV and amperometry. A conventional three-electrode electrochemical system, which consists of a working electrode, a Pt foil auxiliary electrode, and Ag/AgCl reference electrode, was used for all electrochemical experiments.
The crystalline structure of Cu2O–BSA NPs was detected by an X-ray diffractometer (Bruker D8 ADVANCE) with Cu-Kα radiation (λ = 1.5406 Å). A type of H-7650 TEM (Japan) was used to observe the size and morphology of the Cu2O–BSA NPs. A CHI 690 electrochemical workstation (Chenhua, Shanghai, China) was assumed to conduct the electrochemical experiments including CV and amperometry. A conventional three-electrode electrochemical system, which consists of a working electrode, a Pt foil auxiliary electrode, and Ag/AgCl reference electrode, was used for all electrochemical experiments.