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2 2 diphenyl 1 2 4 6 trinitrophenyl hydrazyl

Manufactured by Merck Group
Sourced in United States

2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl is a stable free radical compound used as a reagent in various analytical and research applications. It is a deep purple crystalline solid that exhibits a characteristic absorption spectrum, making it useful in colorimetric and spectrophotometric analyses.

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8 protocols using 2 2 diphenyl 1 2 4 6 trinitrophenyl hydrazyl

1

Quantification of Polyphenolic Compounds

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Standards of catechin (Cat), epicatechin (ECat), procyanidin B2 (PC B2), procyanidin C1 (PC C1), gallic acid (GA), protocatechuic acid (PA), p-hydroxybenzoic acid (p-HBA), cyanidin-3-O-glucoside (Cy-3-Glu), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), sodium acetate, ferric chloride hexahydrate, ferrozine, and ammonium acetate were purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade methyl tert-butyl ether (MTBE) and methanol were purchased from J.T. Baker (Deventer, The Netherlands). All other reagents were of analytical grade and were purchased from Chempur (Piekary Śląskie, Poland). Chromacol PTFE syringe filters (0.2 µm pore size) were purchased from Shim-Pol (Izabelin, Poland). Ultrapure water (resistivity 18.2 MΩ cm), obtained from a Milli-Q purification system (Millipore, Bedford, MA, USA), was used for all analyses.
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2

Comprehensive Biochemical Assay Protocol

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Antimycotic antibiotic solution, decamplmaneh fetal bovine serum, Dulbecco's Modified Eagle Medium (DMED), carbazole reagent, 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), 4-(Dimethylamino)benzaldehyde, phosphate buffer solution (PBS), borax-sulphuric acid mixture, Ehrlich solution, hydrolysate, and dimethyl sulfoxide (DMSO), all of this was obtained from Sigma-Aldrich, USA.
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3

Antioxidant Potential Evaluation

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Gallic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), sodium acetate, ferric chloride hexahydrate, ferrozine, and ammonium acetate were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of analytical grade and were purchased from Chempur (Piekary Slaskie, Poland). Ultrapure water (resistivity 18.2 MΩ cm), obtained from a Milli-Q purification system (Millipore, Bedford, MA, USA), was used for all analyses.
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4

Cassia Seed Extraction and Analysis

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Cassia seeds were obtained from a market in Ya’an, China and dried at a temperature of 45 °C for two days. Subsequently, the dried Cassia seeds were ground to pass through a 60 mesh sieve and stored at −20 °C for further analysis.
Trifluoroacetic acid, rhamnose, glucuronic acid, galacturonic acid, mannose, glucose, galactose, xylose, arabinose, m-hydroxydiphenyl, griess reagent, sodium nitroprusside (SNP), 1-phenyl-3-methyl-5-pyrazolone (PMP), 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide, vitamin C, and butylated hydroxytoluene (BHT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents and chemicals used were of analytical grade.
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5

Identification and Characterization of Daldinia indusiata

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The fruiting body of D. indusiata was bought from the local supermarket in Ya’an, China. The sample was dried, grounded, and sieved through a 60-mesh screen. The powders were stored at −20 °C before use. Carboxymethyl cellulose (CM-cellulose), cholesterol, sodium deoxycholate, sodium taurocholate, sodium glycocholate, sodium cholate, oleic acid, 1-phenyl-3-methyl-5-pyrazolone (PMP), monosaccharide standards, Griess reagent, 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) were obtained from Sigma-Aldrich (Sigma, St. Louis, MO, USA). A free cholesterol assay kit was obtained from Solarbio (Solarbio, Beijing, China).
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6

Extraction and Characterization of Snow Chrysanthemum

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The flower of snow chrysanthemum (C. tinctoria) was purchased from a local market in Ya’an, China. Samples were dried at the temperature of 45 °C for 2 days. Subsequently, the samples were ground to pass through a 60 mesh sieve and stored at −20 °C for further analysis.
Trifluoroacetic acid, rhamnose (Rha), mannose (Man), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), xylose (Xyl), arabinose (Ara), 1-phenyl-3-methyl-5-pyrazolone (PMP), m-hydroxydiphenyl, griess reagent, sodium nitroprusside (SNP), 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), vitamin C, butylated hydroxytoluene (BHT), and 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid (Trolox) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents and chemicals used were of analytical grade.
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7

Peach Antioxidant Properties Extraction

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Peaches (Prunus persica cv. Yanhong) were purchased from an orchard situated in Yaodu District, Linfen City, Shanxi Province. The fruits were picked at commercial mature stage. Peaches with uniform shape, size, and color, as well as no insect pest and mechanical damage, were selected and quickly transported to the laboratory of Shanxi Normal University in open cartons.
CO gas with a purity of 99.99% was purchased from Beijing Huaneng Special Gases Co., Ltd. (Beijing, China). Oxalic acid, gallic acid, rutin, potassium ferricyanide, trichloroacetic acid, and sodium hydroxide (analytical grade) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Analytical-grade 1,1-diphenyl-2-picrylhydrazyl radical, 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl, and 2,6-dichloroindophenol were purchased from Sigma (USA). Other reagents of analytical grade were purchased from Alfa Aesar Company (Tianjin, China).
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8

Characterization of Chrysanthemum Tea Varieties

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Different chrysanthemum teas, including C. tinctoria (snow chrysanthemum tea), C. indicum, C. morifolium ‘Huangju’, C. morifolium ‘Gongju’, and C. morifolium ‘Hangbaiju’, were purchased from a local market in Ya'an, China. Chrysanthemum teas were dried at the temperature of 45 °C for 2 days, and then the dried samples were ground to pass through a 60 mesh sieve, and stored at −20 °C for further analysis.
Trifluoroacetic acid, monosaccharide standards (rhamnose, mannose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose), 1-phenyl-3-methyl-5-pyrazolone (PMP), hydrogen peroxide (H2O2), m-hydroxydiphenyl, griess reagent, vitamin C, sodium nitroprusside (SNP), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), butylated hydroxytoluene (BHT), 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), sodium azide, bovine serum albumin (BSA), and aminoguanidine were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents and chemicals used were of analytical grade.
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