21 030 cvr

Manufactured by Corning

21-030-CVR is a laboratory equipment product. It serves a core function related to laboratory applications, but a detailed description is not available while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Collagenase, by Merck Group (1 mentions) 10 013 cvr, by Corning (1 mentions) C2139, by Merck Group (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Dulbecco phosphate buffered saline (dpbs), by Corning (1 mentions) Trypsin, by Corning (1 mentions)

2 protocols using 21 030 cvr

Isolation and Culture of Primary Murine Neuronal Cells

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The DRG and sciatic nerves were isolated from c57/BL6NJ mice at postnatal day 15 (P15) and transferred to the conical tube containing trypsin of 0.25% (25‐053‐CI, Corning, New York) and 1 mg/mL of collagenase (C2139, Sigma‐Aldrich, Saint Louis, Missouri). The primary tissues were incubated for 30 minutes at 37°C for digestion followed by centrifugation. The cell pellets were suspended in DMEM (10‐013‐CVR, Corning) containing fetal bovine serum of 15% (35‐016‐CV, Corning) and penicillin‐streptomycin of 1% (30‐002‐CI, Corning) after being washed with DPBS (21‐030‐CVR, Corning). The primary cells were cultivated on the coverslips in 35‐mm dishes or 6‐well plates coated with poly‐d‐lysine hydrobromide (P6407, Sigma‐Aldrich) in 5% CO₂ at 37°C.

Won S.Y., Kwon S., Jeong H.S., Chung K.W., Choi B., Chang J.W, & Lee J.E. (2020). Fibulin 5, a human Wharton's jelly‐derived mesenchymal stem cells‐secreted paracrine factor, attenuates peripheral nervous system myelination defects through the Integrin‐RAC1 signaling axis. Stem Cells (Dayton, Ohio), 38(12), 1578-1593.

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Cancer Phosphopeptide Preparation

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MHC-presented phosphopeptides previously identified by mass spectrometry on cancer cells lines and/or primary tumor cells (Supplementary Table S1) were purchased from GenScript. Lyophilized peptides were stored at −80°C until reconstituted to 10mg/ml in DMSO and stored in a desiccator at room temperature. Stock aliquots of individual peptides or combinations of peptides were diluted in Dulbecco’s Phosphate Buffer Solution (DPBS, Corning Cat#21-030-CVR) to 200μg/ml and stored at −80°C.

Lulu A.M., Cummings K.L., Jeffery E.D., Myers P.T., Underwood D., Lacy R.M., Chianese-Bullock K.A., Slingluff CL J.r., Modesitt S.C, & Engelhard V.H. (2021). Characteristics of immune memory and effector activity to cancer-expressed MHC class I phosphopeptides differ in healthy donors and ovarian cancer patients. Cancer immunology research, 9(11), 1327-1341.

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