Taqman probes 5 fluorescein based reporter dye 3 tamra quencher

SPC (>98% purity), EPL (phosphatidylcholine 84.5%, phosphatidyl-ethanolamine 9%, sphingomyelin 3%, lysophosphatidylcholine 3%, lysophosphatidyl-ethanolamine 0.5%) were kindly provided by Doosan Corporation (Seoul, Korea). 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) was obtained from Cayman Chemical (Ann Arbor, MI, USA). Chrysin and the solvents used in this study were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Taqman® Universal master mix, Taqman^® probes (5′-fluorescein based reporter dye; 3′-TAMRA quencher) and the high capacity RNA-to-cDNA kit were purchased from Applied Biosystems (Foster City, CA, USA).

E. cava ethanolic extract (ECE, powder) was provided from Seojin Biotech Co. Ltd. (Suwon, Korea). The total phlorotannin content of ECE used in this study was 67%, of which dieckol was a major constituent (10.6%, w/w) [10 (link)]. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin-streptomycin were purchased from WelGENE Inc. (Daegu, Korea). P. gingivalis LPS and protoporphyrin Ⅸ (SnPP) were obtained from Invivogen (San Diego, CA, USA). A prostaglandin E₂ (PGE₂) ELISA kit and cyclooxygenase (COX) activity assay kit were purchased from Cayman Chemical Co. (Ann Arbor, MI, USA). Taqman^® Universal master mix, Taqman^® probes (5′-fluorescein based reporter dye; 3′-TAMRA quencher) and a high capacity RNA-to-cDNA kit were purchased from Applied Biosystems (Foster City, CA, USA). Antibodies (heme oxygenase-1 (HO-1), nuclear factor erythroid 2-related factor 2 (Nrf-2), NF-κB, β-actin, and TBP) were obtained from Cell Signaling Technology (Danvers, MA, USA). Other reagents were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA).