Hen egg white lysozyme, lysostaphin, nisin, mutanolysin, and melittin were purchased from Sigma Aldrich. The following bacteria were used for antimicrobial activity assays and RNA isolation: Staphylococcus aureus ATCC 6538 (American Type Culture Collection, Manassas, VA, USA), Bacillus cereus ATCC 10876, Enterococcus faecalis ATCC 29212, Streptococcus agalactiae ATCC 27956, Streptococcus dysgalactiae ATCC 27957, Streptococcus equi subsp. zooepidemicus ATCC 43079, Streptococcus iniae KCTC 3657 (Korean Collection for Type Cultures, Daejeon, Korea), Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Salmonella typhimurium ATCC 14028. E. faecalis and streptococci were cultured in brain heart infusion broth (BD Bioscience) because of their slow growth in LB medium. All other bacteria were cultured in LB medium.
Streptococcus iniae kctc 3657
Manufactured by Korean Collection for Type Cultures
Streptococcus iniae KCTC 3657 is a bacterial strain maintained by the Korean Collection for Type Cultures. It is a Gram-positive, catalase-negative, and beta-hemolytic bacterial species. The strain is available for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Mutanolysin, by Merck Group (1 mentions)
Lysostaphin, by Merck Group (1 mentions)
Escherichia coli, by Korean Collection for Type Cultures (1 mentions)
Nisin, by Merck Group (1 mentions)
Hen egg white lysozyme, by Merck Group (1 mentions)
Pseudomonas aeruginosa, by Korean Collection for Type Cultures (1 mentions)
Melittin, by Merck Group (1 mentions)
Bacillus cereus, by American Type Culture Collection (1 mentions)
Brain heart infusion broth, by BD (1 mentions)
Staphylococcus aureus atcc 6538, by American Type Culture Collection (1 mentions)
Enterococcus faecalis, by American Type Culture Collection (1 mentions)
Streptococcus agalactiae, by American Type Culture Collection (1 mentions)
Edwardsiella tarda kctc 12267, by Korean Collection for Type Cultures (1 mentions)
2 protocols using streptococcus iniae kctc 3657
1
Antimicrobial Assays and RNA Isolation
2
Antimicrobial Susceptibility of Aquatic Pathogens
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Streptococcus iniae KCTC 3657, Vibrio anguillarum KCTC 2711 and Edwardsiella tarda KCTC 12267 were purchased from the Korean Collection for Type Cultures (Daejeon, Korea). For antimicrobial susceptibility tests, strains were cultured on Brain Heart Infusion Agar (BHIA) in an incubator at 25 °t for 24 h. Bacterial colonies taken directly from BHIA plates were incubated in Brain Heart Infusion Broth (BHIB) at 25 ° for 24 h. From this culture, a suspension equivalent to a 0.5 McFarland standard in BHIB was prepared.
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