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4 protocols using mold star 30

1

Strain-Conditioned Collagen Sheets

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After extrusion, collagen
sheets were incubated in FFB for 30 min
and subsequently strain conditioned on a custom-made setup (Figure S4). The setup included two clamps, one
fixed and a moveable clamp with a slider on opposing ends to secure
the sheet firmly. The moveable clamp allowed the application of the
desired strain rates. The setup also consisted of a reservoir that
allowed collagen sheets to remain hydrated and submerged in fibrillogenesis
promoting buffer (FPB) during the incubation period (48 h). Sheets
were supported on an elastic polymer strip (Mold Star 30, Smooth-On,
Macungie, PA, U.S.A.) that was molded within an acrylic frame and
was 150 mm long, 20 mm wide, and 1.5 mm thick. Plasma treatment of
the molded elastomeric support substrate for 90 s rendered the surface
hydrophilic and allowed collagen sheets to readily spread. Mounting
the sheet on the strain conditioning setup took around 10 min. After
strain conditioning for 48 h at 37 °C, the collagen sheets were
washed with deionized water, dried, and rehydrated prior to tensile
testing.
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2

Synthesis of Photonic Composite Materials

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SMA, EGDMA, AM, AA, 2,2′-diethoxyacetophenone (DEOP), Py, iron(III) chloride hexahydrate (FeCl3·6H2O), 4′,6-diamidino-2-phenylindole dihydrochloride, and all n-alkanes (CnH2n+2, n = 16, 18, 26, 28, 36, 44, and 50) were purchased from Sigma-Aldrich. Aluminum sol (AlOOH, pseudoboehmite, solid content 20%) was purchased from Suzhou Haipu Hydraulic Equipment Co. Poly(ethylene glycol)–block–poly (propylene glycol)–block–poly (ethylene glycol) [PEG-PPG-PEG; number-average molecular weight (Mn) ~ 12,600, as an emulsifier] was purchased from Aladdin Co. N,N′-Bis(2,6-diisopropylphenyl)-1,6,7,12-tetraphenoxy-3,4,9,10-perylenetetracarboxylic diimide (Perylene Red) was purchased from Tokyo Chemical Industry. All the chemicals were used as purchased without further purification. Ecoflex 30 and Mold Star 30 were purchased from Smooth-On Inc., USA. Ni-Cr resistive wire with a diameter of 0.1 mm was bought from www.1688.com.
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3

Fabrication and Characterization of Vaginal Tissue Phantoms

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Multiple-part elastomers were used to fabricate the vaginal tissue phantoms, which are described in detail by Chanda et al. [13 ,14 (link),15 ,16 (link),17 (link),18 ,19 (link),20 (link),21 ]. For this study, a two-part elastomer material with shore hardness 10 (Ecoflex® 0010, Smooth-On, Inc., Macungie, PA, USA) and another two-part elastomer with shore hardness 30A (Mold Star 30, Smooth-On, Inc.) were procured and mixed to obtain a four-part mixture. The resulting mixture comprised of 35 wt % of Shore 30A (part A), 35 wt % of Shore 30A (part B), 15 wt % of Shore 10 (part A), and 15 wt % of Shore 10 (part B). Twenty-seven test coupons were casted (Figure 2a) with this mix ratio in a mold, with coupon dimensions of 5 cm × 1 cm × 3 mm. Uniaxial mechanical tests were conducted on the test coupons using a tensile testing machine (MTS Criterion, Model 42, MTS Systems Corporation, Eden Prairie, MN, USA) at a test rate of 0.08 mm/s [13 ,14 (link),15 ], the results of which are summarized in Figure 2b. The stress versus stretch results from the tests were compared with the average mechanical property of excised human vaginal tissue samples in literature measured by Calvo et al. at a 0.08 mm/s test rate [22 (link)] (Figure 2b).
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4

Multi-material 3D Printed Bioinspired Disc

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We fabricated the main disc by using a multi-material 3D printer Objet Connex C3 (Stratasys Ltd, Eden Prairie, MN, USA) (Fig. S6B). The main disc's components are 3D printed with different materials. For example, the material of soft lip around the disc base edge and soft tissue of lamellae is TangoPlus FLX930 (Stratasys Ltd, Eden Prairie, MN, USA). The material of rigid lamellae is VeroWhitePlus RGD835 (Stratasys Ltd, Eden Prairie, MN, USA). The flexible base is fabricated by mixed materials of TangoPlus and VeroWhitePlus, RGB 8505 (Stratasys Ltd, Eden Prairie, MN, USA) (Fig. S6B-D). By molding and casting silicone rubber (Ecoflex 10, Smooth-on, Inc, Easton Pa, USA), we fabricated the soft layer (with a thickness of 800 μm) on top of the disc lip (Fig. S6A). The spinules were fabricated by laser-cutting a 200 μm stainless steel plate. Then, the spinules were inserted into the lamellae (Fig. S6D). The lamellae actuator with micro-channels was printed by a multi-material 3D printer using silicone materials (ACEO@ Silicone GP, Wacker Chemie AG, Germany) (Fig. S6E). The bending actuator was fabricated by molding/casting silicone rubber (Mold Star 30, Smooth-on, Inc, Easton Pa, USA) with fiber reinforcements (Fig. S7). Finally, different layers were bonded by silicone rubber adhesive (Sil-Poxy, Smooth-on, Inc, Easton Pa, USA) (Fig. S6F).
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