sheets were incubated in FFB for 30 min
and subsequently strain conditioned on a custom-made setup (
fixed and a moveable clamp with a slider on opposing ends to secure
the sheet firmly. The moveable clamp allowed the application of the
desired strain rates. The setup also consisted of a reservoir that
allowed collagen sheets to remain hydrated and submerged in fibrillogenesis
promoting buffer (FPB) during the incubation period (48 h). Sheets
were supported on an elastic polymer strip (Mold Star 30, Smooth-On,
Macungie, PA, U.S.A.) that was molded within an acrylic frame and
was 150 mm long, 20 mm wide, and 1.5 mm thick. Plasma treatment of
the molded elastomeric support substrate for 90 s rendered the surface
hydrophilic and allowed collagen sheets to readily spread. Mounting
the sheet on the strain conditioning setup took around 10 min. After
strain conditioning for 48 h at 37 °C, the collagen sheets were
washed with deionized water, dried, and rehydrated prior to tensile
testing.