Sarcometic contractions and calcium transients were measured as described previously [41 (link)]. The cardiomyocytes from the left ventricle (n = 16–23 in each group) were incubated with 2-μmol/L-Fura-2-AM Tyrode solution for 20 min and then washed with Tyrode solution repeatedly. The sarcomeric contractions and calcium transients were recorded in the Tyrode solution (control), 0.1-μmol/L-NPW Tyrode solution, and 0.1-μmol/L-NPB solution at 37 °C using the Ionoptix HyperSwitch Myocyte Calcium and Contractility System (IonOptix LLC, Westwood, CA, USA). The composition of the Tyrode solution was as follows (in mmol/L): NaCl 137, KCl 4.5, MgCl2 1, CaCl2 2, glucose 10, HEPES 5, and pH adjusted to 7.4 with NaOH. All chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). During the measurement, the cells were stimulated with a field stimulator (MyoPacer Field Stimulator, IonOptix LLC, Westwood, CA, USA) at a frequency of 1 Hz. The offline analysis was performed with IonWizard 6.5 software (IonOptix LLC, Westwood, CA, USA).
Hyperswitch myocyte calcium and contractility system
Manufactured by IonOptix
Sourced in United States
The IonOptix HyperSwitch Myocyte Calcium and Contractility System is a laboratory equipment designed to measure and analyze the calcium dynamics and contractility of isolated cardiac or skeletal muscle cells. It provides real-time monitoring and recording of cellular calcium levels and contractile properties.
Automatically generated - may contain errors
2 protocols using hyperswitch myocyte calcium and contractility system
1
Cardiac Calcium Dynamics and Contractility Assay
2
Contractility and Calcium Dynamics in CMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In order to evaluate functional properties of CMCs cellular contractility and calcium transients were analyzed. For the experiment CMCs were transferred from cultivation media to normal calcium (2 mmol/l) Tyrode solution. CMCs sarcomeric contractions and calcium transients were measured with Ionoptix HyperSwitch Myocyte Calcium and Contractility System (IonOptix LLC, Westwood, USA), with the Sarclen sarcomere length acquisition module. Cells were loaded with Fura-2 (Molecular Probes, Invitrogen, USA). For stock solution Fura-2-am powder was dissolved in DMSO (Sigma-Aldrich, USA) to reach final concentration of 1 mmol/l. Cells were incubated for 20 min in normal calcium Tyrode solution with 2 μmol/l Fura-2-am and then repeatedly washed with normal calcium Tyrode solution. After 20 min of incubation, measurements followed. Measurements were performed in normal Tyrode solution at 37±0.5 °C. Cells were stimulated with field stimulator (MyoPacer Field Stimulator, IonOptix LLC, Westwood, USA) at 1 Hz.
For offline analysis of sarcomeric contractions and calcium transients the IonWizard 6.5 software (IonOptix LLC, Westwood, USA) was used.
For offline analysis of sarcomeric contractions and calcium transients the IonWizard 6.5 software (IonOptix LLC, Westwood, USA) was used.
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