Mettler dsc 823e

Thermograms of CFA, βCD, ARG and complexes were recorded on a DSC analyzer (Mettler DSC 823E Mettler Toledo Pvt. Ltd, Switzerland). A sample (5 mg) was placed in an aluminum pan and heated under a nitrogen atmosphere (flow rate 100 mL/min) at a heating rate of 10 °C/min over the temperature range of 30–300 °C. The thermal behaviour of samples was recorded and studied.

A Mettler DSC 823e (Mettler Toledo, Spain) was used for the DSC measurements. Prior to analysis, indium of purity ≥99.95% (Fluka, Switzerland) was used to calibrate the device. Accurately weighted mass of 1.0–2.0 mg of cetyl palmitate, octyl methoxycinnamate (Parsol® MCX), or an equivalent amount of lipid formulated as hybrid nanoparticles, were weighted in aluminium pan of 40.0 μl, which were then cold sealed. An empty pan was used as reference. Heating curves for the bulk materials were recorded with a scan rate of 5.0ºC/min from 25.0 to 200.0 °C and then cooled down to 25.0 °C, using liquid nitrogen. For the analysis of SLN formulations, heating curves were recorded from 25.0 °C to 85.0 °C following cooling down to 25.0 °C, at a scan rate of 5.0ºC/min. The Mettler STARe V 9.01 DB software (Mettler Toledo, Spain) was used for the collection and storage of the data of peak areas.