Prism graph pad 7

All experiments were performed in triplicates and repeated at least twice in each cell model (N≥6). PRISM GraphPad 7 was used to perform all the statistical analyses presented. Two group comparisons were analyzed for variation and significance using a two-tailed, type 1 t-test and p values lower than 0.05 were considered significant and symbolized by an asterisk in the graphs. One-way or Two-way ANOVA and Tukey post hoc test was used to analyze the relationships when comparing multiple variables, with p values lower than 0.05 considered to be statistically significant. All data shown are representative of means ± S.D. of triplicate results unless otherwise specified.
Additional information about polymer synthesis, primers used for qRT-PCR (Supplemental table S1, S2 and S3), cloning (Supplemental table S4), lentiviral constructs (Supplemental Table S5), antibodies (Supplemental table S6), miRNA array resuls (Supplemental table S7) can be found in the supplemental materials.

All statistical analyses were conducted using Prism GraphPad 7 and R-studio. We conducted all correlational analysis in Prism 7 computing two-tailed Pearson correlation coefficients with 95% confidence intervals. Unlike our previous work, we did not choose an arbitrary cutoff to be used for a binary classification of “healthy” and “depressed” as we did not recruit a clinically assessed sample. Instead, multiple linear regressions were used to assess the influence of Beck Depression Inventory-II (on a continuous basis) on relationships between behavior, fMRI, and diffusion measures and were conducted in R-studio. Bootstrap analysis were conducted in R-studio using the Boot package with 1000 repetitions. 95% confidence intervals are reported. Statistical values were considered significant at an alpha level of 0.05. Since we only tested targeted hypotheses and used a priori planned comparisons, correction for multiple comparisons was not necessary (Rothman, 1990 (link); Saville, 1990 ).