Hrp conjugated anti rabbit and anti goat secondary antibodies

Antibodies against EGFR, Akt, MEK1/2 and tubulin were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against cleaved PARP and HIF-1α were purchased from BD Biosciences (San Jose, CA, USA). Antibodies against IGF-1R and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HRP-conjugated anti-rabbit and anti-goat secondary antibodies were purchased from GeneTex (Irvine, CA, USA). An anti-Hsp90 antibody was purchased from Enzo Life Science (Farmingdale, NY, USA). Ni-NTA agarose was purchased from Invitrogen (Carlsbad, CA, USA). ATP-agarose was acquired from Innova Biosciences (Cambridge, UK). The first-strand cDNA synthesis kit was purchased from Dakara Korea Biomedical, Inc. (Seoul, Republic of Korea). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI), crystal violet, and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise specified.

Antibodies against pIGF1R (Y1135/6), IGF1R, pSrc (Y416), Src, phosphor-tyrosine (pTyr), pMEK1/2, MEK1/2, pAkt (S473), Akt, and cleaved caspase 3 were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against cleaved PARP and FAK  were purchased from BD Biosciences (San Jose, CA, USA). A primary antibody against pFAK (Y576/577) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Primary antibodies against IGF1R, IR, and actin and the horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HRP-conjugated anti-rabbit  and anti-goat secondary antibodies were purchased from GeneTex (Irvine, CA, USA). Linsitinib and dasatinib were purchased from Selleckchem (Houston, TX, USA) or LC Laboratories (Woburn, MA, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise specified.