Lamp reagents

All LAMP reagents were purchased
from New England Biolabs (Ipswich, MA), and all primers were ordered
from Integrated DNA Technologies (Coralville, IA), unless otherwise
mentioned. Calcein, MnCl₂, as well as acids were purchased
from Sigma-Aldrich (St. Louis, MO). Lysozyme, SYBR Green, and culture
media were obtained from ThermoFisher Scientific (San Jose, CA). Track-etched
PC membranes, PES membranes, and Nylon membranes were purchased from
Sterlitech Corporation (Kent, WA). Sylgard 184 silicon elastomer kit,
consisting of a prepolymer base and a curing agent, was obtained from
Dow Corning (Midland, MI).

All LAMP reagents were purchased
from New England Biolabs (Ipswich, MA), and the primers were from
Integrated DNA Technologies (Coralville, IA) unless otherwise mentioned.
Calcein and MnCl₂ were purchased from Sigma-Aldrich (St.
Louis, MO). Culture media were obtained from ThermoFisher Scientific
(San Jose, CA). Track-etched polycarbonate membranes were purchased
from Sterlitech Corporation (Kent, WA) and GVS Filter Technology (USA).
Sylgard 184 silicon elastomer kit consisting of a prepolymer base
and a curing agent was obtained from Dow Corning (Midland, MI). The
PDMS films were prepared by mixing their precursor and curing agent
at a ratio of 10:1 and cured at 75 °C for 1.5 h.^{28 (link)}