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Mes sa cell line

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The MES-SA cell line is a human uterine sarcoma cell line derived from a malignant tumor. It is a well-characterized model system commonly used in cancer research and drug development studies.

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2 protocols using mes sa cell line

1

Evaluating Effects of HDAC Inhibitors on Leiomyoma Cells

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The source and culture condition of human leiomyoma cell line (HuLM), uterine smooth muscle (UTSM) cell line, SK-UT1 cell line (ATCC, Manassas, VA, USA), and MES-SA cell line (ATCC, Manassas, VA, USA) were described previously [27 (link)].
Class I HDAC inhibitor Tucidinostat was purchased from Selleck Chemical (Cat# S8567, Houston, TX, USA). DL-sulforaphane was purchased from Sigma-Aldrich (Cat# S4441, Saint Louis, MO, USA). The range of doses tested was 1–25 µM.
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2

Evaluation of Compound Activity against Multi-Drug Resistant Tumor Cell Line

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Example 22

The activity of example compounds to multi-drug resistant tumor cell line MES-SA was evaluated.

Experimental materials: DMEM high-glucose cell culture media (Hyclone Company), fetal calf serum (FBS) (Gibco Company), penicillin and streptomycin were purchased from North China Pharmaceutical Co., Ltd, phosphate buffer saline (PBS) was purchased from Gibco Company, Cell Titer-Glo® cell viability detection reagent was purchased from Promega Company, pancreatin and dimethylsulfoxide (DMSO) were products of Sigma Company. MES-SA cell line (human uterine sarcoma cell line) was purchased from ATCC. MRC5 cell (human embryonic lung fibroblast) was purchased from ATCC.

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