Elf18

Leaf tissue was collected from 4-week old Arabidopsis plants that were syringe-infiltrated with 10 µM flg22 (Genscript) or 10 µM elf18 (Genscript).
Pathogen treatments were performed using 4-week old plants that were syringe-infiltration with Psm ES4326/avrRpm1 (OD_600nm = 0.1), spray inoculated with Pst DC3000 (OD_600nm = 0.2, 0.02% Silwet L-77) or Pst DC3118 (OD_600nm = 0.2, 0.02% Silwet L-77). Untreated root, shoot leaf, shoot, and flower tissues were collected to determine the basal levels of tarnscripts. We extracted total RNA from the collected samples using RiboZol (AMRESCO). Possible genomic DNA contamination was eliminated using DNase I (Ambion). Formaldehyde agarose gel preparation, quantification, electrophoresis and samples prepration/loading were done following the RNeasy Plant Mini® Kit QIAGEN protocol. SuperScript III first-strand RT-PCR kit (Invitrogen) was used to convert mRNA into cDNA. We used GoTaq qPCR Master Mix (Promega) to perform qRT-PCR using gene-specific primers in a RealPlex S MasterCycler (Eppendorf). Primers used in this study are listed in the Supplementary Table 1.

4-week old Arabidopsis plants were syringe-infiltrated with 10 μM flg22 (Genscript) or 10 μM elf18 (Genscript) and samples were collected at designated time points. To induce effector-triggered immunity, 4-week old plants were syringe-infiltrated with P. syringae pv. maculicola ES4326/AvrRpm1 (OD_600 nm = 0.1) and samples were collected at designated time points. 4-week old plants were spray inoculated with DC3000 (OD_600 nm = 0.2, 0.02% Silwet L-77) or DC3118 (OD_600 nm = 0.2, 0.02% Silwet L-77) and samples were collected at designated time points.
Total RNA was extracted from collected samples using RiboZol (AMRESCO) and genomic DNA contamination was removed using DNase I (Ambion). mRNA was converted into cDNA using the SuperScript III first-strand RT-PCR kit (Invitrogen). Gene expression analysis was conducted with GoTaq qPCR Master Mix (Promega) using gene-specific primers in a RealPlex S MasterCycler (Eppendorf). Primers used for this study are listed in the Supplementary Table S7.