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Cd73 efluor450

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The CD73 eFluor450 is a fluorescently labeled antibody that binds to the CD73 cell surface protein. CD73 is an enzyme involved in the extracellular metabolism of adenosine monophosphate (AMP) to adenosine. The eFluor450 fluorescent dye allows for the detection and analysis of CD73-expressing cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Cd45 fitc, by BD (2 mentions) Cd45 amcyan, by BD (2 mentions) Cd19 pe cy5, by BD (2 mentions) Cd73 fitc, by Thermo Fisher Scientific (2 mentions) Cd39 pe cy7, by Thermo Fisher Scientific (2 mentions) Lsr 2 fortessa cytometer, by BD (2 mentions) Cd4 rm4 5 percpcy5, by BD (2 mentions) Cd8 53 6.7 bv650, by BD (2 mentions) Cd11c n418 pe, by BD (2 mentions) Foxp3 fjk16s pe, by BD (2 mentions) Fr4 pe cy7, by Thermo Fisher Scientific (2 mentions) Pdl1 percp efluor710, by Thermo Fisher Scientific (2 mentions) Mhc 2 1 ab efluor450, by Thermo Fisher Scientific (2 mentions) Igm fab apc, by Jackson ImmunoResearch (2 mentions) Rat igg1 hrpn percp cy5.5 isotype, by Cytek Biosciences (2 mentions) Rat igg2a 2a3 violetfluor450 isotype, by Cytek Biosciences (2 mentions) Cd45 apc cy7, by BD (1 mentions) Cd4 percp, by BD (1 mentions) Igm pe, by Southern Biotech (1 mentions) Cd19 pe cy7, by BioLegend (1 mentions)

5 protocols using cd73 efluor450

1

Multicolor Flow Cytometry Panel

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Antihuman: p-BTK (pY223) PE, CD19 PE-Cy5, IgM PE, CD45 AmCyan and CD45 FITC (Becton Dickinson, USA), CD39 PE-Cy7, CD73 FITC, CD73 eFluor450, CD21 FITC and CD23 APC (eBioscience, USA). Anti-mouse: B220 FITC, CD4 PerCP, CD45 APC-Cy7 (BD-Pharmingen, USA), IgM PE (Southern Biotech, USA) and CD3e eFlour450, CD39 PE-Cy7, IgD FITC, IgM APC (eBioscience, USA), IgD APC-Cy7, CD73 PacificBlue, CD19 PE-Cy7 and CD8a PE (BioLegend, USA). All human mAbs were titrated using normal PBMC to establish optimal dilution.
Jeske S.S., Brand M., Ziebart A., Laban S., Doescher J., Greve J., Jackson E.K., Hoffmann T.K., Brunner C, & Schuler P.J. (2020). Adenosine-producing regulatory B cells in head and neck cancer. Cancer Immunology, Immunotherapy, 69(7), 1205-1216.
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2

Multicolor Flow Cytometry for Treg Immunophenotyping

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Multicolour flow cytometry was used to assess the immunophenotype of Tregs. Antibodies against the following molecules were provided by: CD3 BV650 (BD Biosciences), CD8 BV450 (BD Biosciences), CD4 APC-H7 (BD Biosciences), CD25 PerCPCy5.5 (BD Biosciences), CD73 eFluor 450 (eBioscience™), PD-1 BV 650 (Biolegend). Dead cells were stained by LIVE/DEAD® Fixable Blue Dead Cell Stain Kit (Life Technology). For intracellular staining, cells were fixed and permeabilized using the FoxP3/Transcription Factor Staining Buffer Set (eBioscience), and then stained with FoxP3 Alexa Fluor 488 (eBioscience), IL13-PE (eBioscience) and Granzyme B APC (BioLegend). Appropriate isotype controls were used to set the quadrants and to evaluate background staining. Cells were analysed using an LSRII flow cytometer with BD FACSDIVA software.
Faridar A., Thome A.D., Zhao W., Thonhoff J.R., Beers D.R., Pascual B., Masdeu J.C, & Appel S.H. (2020). Restoring regulatory T-cell dysfunction in Alzheimer’s disease through ex vivo expansion. Brain Communications, 2(2), fcaa112.
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3

Multi-color Flow Cytometry Panel for Immune Profiling

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Antibodies for flow cytometry and histology include CD3 PE, CD4 (RM4-5) PerCPCy5.5, CD8 (53-6.7) BV650, CD11c (N418) PE, FOXP3 (FJK16S) PE, were purchased from BD Biosciences (San Jose, CA); NK1.1 (PK136), CD11b (M1/70), CD11c (N418), B220 (RA3-6B2), and F4/80 in APC-eFluor780; PD1 (J43) FITC, CD73 eFluor450, FR4 PE-Cy7, PDL1 PerCP-eFluor710, MHC-II I-Ab eFluor450, IL7R PE, and all ELISpot antibodies were purchased from eBiosciences (San Diego, CA), and IgM (Fab′) APC was purchased from Jackson Immunoresearch (West Grove, PA). Rat IgG1 (HRPN) PerCP-Cy5.5 Isotype and Rat IgG2a (2A3) violetFluor450 Isotype were purchased from Tonbo Biosciences (San Diego, CA). Cells from enriched fractions were analyzed on an LSR-II Fortessa cytometer (BD Biosciences, San Jose CA) and data was analyzed in FlowJo (Treestar, Ashland OR). Intracellular cytokine staining was done using BD Cytofix/Cytoperm reagents and protocols (BD Biosciences, San Jose CA). Anti-IL10 (JES5-16E3, PE, eBiosciences), TGFB1(LAP) (TW7-16B4, ef710, eBiosciences), IFNγ (XMG1.2, BV421, BD Horizon), and TNFα (MP6-XT22, AF488, eBiosciences) were stained by this procedure.
Manlove L.S., Berquam-Vrieze K.E., Pauken K.E., Williams R.T., Jenkins M.K, & Farrar M.A. (2015). Adaptive immunity to leukemia is inhibited by cross-reactive induced regulatory T cells. Journal of immunology (Baltimore, Md. : 1950), 195(8), 4028-4037.
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4

Multiparameter Flow Cytometry Analysis

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Antibodies for flow cytometry include CD3 PE, CD4 (RM4-5) PerCPCy5.5, CD8 (53-6.7) BV650, CD11c (N418) PE, FOXP3 (FJK16S) PE, CD80 (16-10A1) APC, CD86 (GL1) PE-Cy7, CD19 BV605, B220 (RA3-6B2) Horizon V500, IFNgamma (XMG1.2) BV650, LAP (TW7-16B4) PE, TNF alpha (MP6-XT22) BV421, IL17A (TC11-18H10) AlexaFluor488, and PSGL1 (2PH1) BV421 purchased from BD Biosciences (San Jose, CA); NK1.1 (PK136), CD11b (M1/70), CD11c (N418), B220 (RA3-6B2), and F4/80 in APC-eFluor780; PD1 (J43) FITC, CD73 eFluor450, FR4 PE-Cy7, PDL1 PerCP-eFluor710, MHC-II I-Ab eFluor450, IL10 (JESS-16E3) PE, Granzyme B (NGZB) PE-Cy7, GARP (YGIC86) eFluor450, and all ELISpot antibodies were purchased from eBiosciences (San Diego, CA), and IgM (Fab′) APC was purchased from Jackson Immunoresearch (West Grove, PA). Rat IgG1 (HRPN) PerCP-Cy5.5 Isotype and Rat IgG2a (2A3) violetFluor450 Isotype were purchased from Tonbo Biosciences (San Diego, CA). Cells from enriched fractions were analyzed on an LSR-II Fortessa cytometer (BD Biosciences, San Jose CA) and data was analyzed in FlowJo (Treestar, Ashland OR).
Manlove L.S., Schenkel J.M., Manlove K.R., Pauken K.E., Williams R.T., Vezys V, & Farrar M.A. (2016). Heterologous vaccination and checkpoint blockade synergize to induce anti-leukemia immunity. Journal of immunology (Baltimore, Md. : 1950), 196(11), 4793-4804.
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5

Multiparametric Flow Cytometry Panel

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The following anti-human monoclonal antibodies (mAbs) were used for flow cytometry: CD4 Alexa Fluor ® 700, CD8 APC, CD39 PE-Cy7, CD73 FITC, and CD73 eFluor450, CCR7 (CD197) PE-Cy7, PD-1 (CD279) PE (eBioscience); CD19 PE-Cy5, CCR7 PE-CF594, CD45 AmCyan and CD45 FITC (Becton Dickinson), CD3 APC-H7, CD25 FITC, CD25 PE (MACS Miltenyi). All mAbs were titrated using PBMC of healthy donors to establish optimal dilution.
Jeske S.S., Weissinger S.E., Veit J.A., Brunner C., Huber U., Theodoraki M.N., Hoffmann T.K., Schuler P.J, & Doescher J. (2019). Treatment-induced changes of lymphocyte subsets in patients with adenoid cystic carcinoma of the head and neck. European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 276(5).
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