Sensimix sybr mix

Manufactured by Meridian Bioscience

Sourced in Australia

SensiMix SYBR mix is a ready-to-use qPCR master mix that contains all the necessary components for real-time PCR amplification and detection using SYBR Green I dye. The mix includes a proprietary DNA polymerase, dNTPs, MgCl2, and SYBR Green I, optimized for sensitive and reproducible quantification of DNA targets.

Automatically generated - may contain errors

Lab products found in correlation

Quantitect reverse transcription kit, by Qiagen (7 mentions) Mirneasy mini kit, by Qiagen (5 mentions) Trizol reagent, by Thermo Fisher Scientific (5 mentions) Rotorgene 6000, by Meridian Bioscience (4 mentions) Improm 2 reverse transcription system kit, by Promega (3 mentions) Hybond n nitrocellulose membrane, by GE Healthcare (3 mentions) Odyssey infrared imaging system, by LI COR (3 mentions) Mirneasy rna extraction kit, by Qiagen (2 mentions) Rneasy miniprep kit, by Qiagen (1 mentions) Genejet genomic dna purification kit, by Thermo Fisher Scientific (1 mentions) Rotor gene q, by Qiagen (1 mentions) Random primer, by Promega (1 mentions) Turbo dna free kit, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Protein a agarose beads, by Repligen (1 mentions) Enhanced chemiluminescence, by GE Healthcare (1 mentions) Rnaseout inhibitor, by Thermo Fisher Scientific (1 mentions) Genejet pcr purification kit, by Thermo Fisher Scientific (1 mentions) Miniopticon system, by Bio-Rad (1 mentions) Fastprep fp120 system, by Qbiogene (1 mentions)

Close spelling variants of this product

IQ SensiMix SYBR master mix SensiMix SYBR No-ROX mix SensiMix SYBR master mix SensiMix SYBR

13 protocols using sensimix sybr mix

Quantifying Transcriptional Changes in ESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated from ESCs or RA-treated ESCs using either TRIzol reagent (Thermo scientific) for Fli1 enhancer deletions or the RNeasy Miniprep kit (Qiagen) for the Hoxb3 enhancer deletion and Med13/13l^fl/fl cells following manufacturer's instructions and cDNA was synthesized from 400 ng RNA using random primers and ImProm-II Reverse Transcription system kit (Promega). RT-qPCR was performed using SensiMix SYBR mix (Bioline) with the primers indicated in Table 2. Ikbkap and Bcor genes were used as controls for Fli1E and Hoxb3E deletions, respectively and Rad23b was used for Med13/13l^fl/fl cells. For each clone mean expression levels across 3 (Fli1E deletion) or 2 (Hoxb3E deletion) technical replicates were calculated. Of these means the mean expression level was calculated across all clones for deletion and corresponding wild-type clones. The expression levels were then normalized to wild-type.

Feldmann A., Dimitrova E., Kenney A., Lastuvkova A, & Klose R.J. (2020). CDK-Mediator and FBXL19 prime developmental genes for activation by promoting atypical regulatory interactions. Nucleic Acids Research, 48(6), 2942-2955.

+ Open protocol

+ Expand

Mitochondrial RNA Expression Analysis

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was isolated from total hearts or heart mitochondria, and northern blotting and qRT-PCR were performed as described previously (18 (link), 41 (link)). RNA was isolated using the miRNeasy Mini Kit (Qiagen) incorporating an on-column RNase-free deoxyribonuclease (DNase) digestion to remove all DNA. RNA (3 μg) was resolved on 1.2% agarose formaldehyde gels, then transferred to 0.45-μm Hybond-N⁺ nitrocellulose membrane (GE Lifesciences), and hybridized with biotinylated oligonucleotide probes specific to mouse mt-mRNAs, rRNAs, and tRNAs (42 (link)). Hybridizations were carried out overnight at 50°C in 5× SSC, 20 mM Na₂HPO₄, 7% SDS, and heparin (100 μg ml⁻¹), followed by washing. The signal was detected using streptavidin-linked infrared-labeled antibody [diluted 1:2000 in 3× SSC, 5% SDS, and 25 mM Na₂HPO₄ (pH 7.5)] using the Odyssey Infrared Imaging System (Li-Cor). Complementary DNA (cDNA) was prepared using the QuantiTect Reverse Transcription Kit (Qiagen) and used as a template in the subsequent PCR that was performed using a Corbett Rotor-Gene 6000 instrument with SensiMix SYBR mix (Bioline) and normalized to 18S rRNA.

Rudler D.L., Hughes L.A., Perks K.L., Richman T.R., Kuznetsova I., Ermer J.A., Abudulai L.N., Shearwood A.M., Viola H.M., Hool L.C., Siira S.J., Rackham O, & Filipovska A. (2019). Fidelity of translation initiation is required for coordinated respiratory complex assembly. Science Advances, 5(12), eaay2118.

+ Open protocol

+ Expand

DNA Extraction and Real-Time PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was extracted from cells using a GeneJET Genomic DNA purification kit according to the manufacturer’s instructions (Fermentas, Thermo Fisher Scientific). Real-time PCR was conducted on 100 ng of DNA using previously described primers (5 ). Amplification was conducted using a Rotor-Gene-Q (Qiagen) using SensiMix SYBR mix (Bioline).

Lee R.G., Balasubramaniam S., Stentenbach M., Kralj T., McCubbin T., Padman B., Smith J., Riley L.G., Priyadarshi A., Peng L., Nuske M.R., Webster R., Peacock K., Roberts P., Stark Z., Lemire G., Ito Y.A., Boycott K.M., Geraghty M.T., van Klinken J.B., Ferdinandusse S., Zhou Y., Walsh R., Marcellin E., Thorburn D.R., Rosciolli T., Fletcher J., Rackham O., Vaz F.M., Reid G.E, & Filipovska A. (2022). Deleterious variants in CRLS1 lead to cardiolipin deficiency and cause an autosomal recessive multi-system mitochondrial disease. Human Molecular Genetics, 31(21), 3597-3612.

+ Open protocol

+ Expand

Quantitative RNA Analysis of Pancreatic Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

The tissues were snap-frozen and stored at –80°C before RNA isolation, while pancreatic islets were isolated fresh using collagenase digestion as described below. RNA was isolated from tissues and pancreatic islets of Langerhans using the miRNeasy Mini kit (Qiagen) using an on-column RNase-free DNase digestion, and the isolated RNA (4 μg) was resolved on 1.2% agarose formaldehyde gels. Northern blotting was carried out as described previously (13 (link)) by transferring the RNA to 0.45 μm of Hybond-N⁺ nitrocellulose membrane (GE Lifesciences) and hybridizing using biotinylated oligonucleotide probes as described before (13 (link)). Streptavidin-linked infrared-labeled antibody [diluted 1:10,000 in 3× SSC, 5% SDS, and 25 mM Na₂HPO₄ (pH 7.5)] was used to detect the signal and visualize the signal using the Odyssey Infrared Imaging System (LI-COR Biosciences). The QuantiTect Reverse Transcription Kit (Qiagen) was used to prepare complementary DNA (cDNA) that was used as a template for PCR. PCRs were performed using a Corbett Rotorgene 6000 using SensiMix SYBR mix (Bioline) and normalized to 18S rRNA.

Rossetti G., Ermer J.A., Stentenbach M., Siira S.J., Richman T.R., Milenkovic D., Perks K.L., Hughes L.A., Jamieson E., Xiafukaiti G., Ward N.C., Takahashi S., Gray N., Viola H.M., Hool L.C., Rackham O, & Filipovska A. (2021). A common genetic variant of a mitochondrial RNA processing enzyme predisposes to insulin resistance. Science Advances, 7(39), eabi7514.

+ Open protocol

+ Expand

Isolation and Quantification of RNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated using TRIzol reagent (Thermo scientific) following manufacturer’s instructions and cDNA was synthesized from 400 ng RNA using random primers and ImProm-II Reverse Transcription system kit (Promega). RT-qPCR was performed using SensiMix SYBR mix (Bioline). Idh1 and Atp6IP1 genes were used as house-keeping controls.

Dimitrova E., Kondo T., Feldmann A., Nakayama M., Koseki Y., Konietzny R., Kessler B.M., Koseki H, & Klose R.J. (2018). FBXL19 recruits CDK-Mediator to CpG islands of developmental genes priming them for activation during lineage commitment. eLife, 7, e37084.

+ Open protocol

+ Expand

Quantification of Gene Expression by qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was isolated from cell nuclei using either TRIzol reagent (Invitrogen) or RNeasy Mini Kit (Qiagen). Genomic DNA was digested with the TURBO DNA-free Kit (Invitrogen). cDNA was synthesized from 400 ng of RNA using ImProm-II Reverse Transcription system kit and random primers (Promega). Quantitative PCR was performed in technical duplicates using SensiMix SYBR mix (Bioline) and primers listed in Supplementary Table 2. The U6 snRNA gene was used as an internal control.

Dobrinić P., Szczurek A.T, & Klose R.J. (2021). PRC1 drives Polycomb-mediated gene repression by controlling transcription initiation and burst frequency. Nature structural & molecular biology, 28(10), 811-824.

+ Open protocol

+ Expand

Mitochondrial Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The transcript abundance of mitochondrial genes and pre-processed junctions was measured on RNA isolated from cells using the miRNeasy RNA extraction kit (Qiagen). Levels of AUH mRNA were measured from RNA isolated from cells or purified mitochondria. Complementary DNA (cDNA) was prepared using the QuantiTect Reverse Transcription Kit (Qiagen) and used as a template in the subsequent PCR that was performed using a Corbett Rotorgene 6000 using SensiMix SYBR mix (Bioline) and normalized to 18S rRNA, U2 snRNA and HPRT1 mRNA.

Richman T.R., Davies S.M., Shearwood A.M., Ermer J.A., Scott L.H., Hibbs M.E., Rackham O, & Filipovska A. (2014). A bifunctional protein regulates mitochondrial protein synthesis. Nucleic Acids Research, 42(9), 5483-5494.

+ Open protocol

+ Expand

PCGF2 and RYBP ChIP-qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols

For PCGF2 and RYBP ChIP-qPCR, double-crosslinked chromatin was prepared as described above. Chromatin was diluted and pre-cleared using protein A agarose beads (Repligen) and incubated overnight with either anti-PCGF2 (in house, 5 μl), or anti-RYBP (Millipore, AB3637, 5 μl) antibody. ChIP and Input DNA were purified as described above. ChIP-experiments were carried out in biological triplicates, and quantitative PCR was performed in technical duplicates using SensiMix SYBR mix (Bioline) and primers listed in Supplementary Table 2.

+ Open protocol

+ Expand

Liver and Skeletal Muscle RNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was isolated from total liver or skeletal muscle using the miRNeasy Mini kit (Qiagen) incorporating an on-column RNase-free DNase digestion to remove all DNA. Complementary DNA (cDNA) was prepared using the QuantiTect Reverse Transcription Kit (Qiagen) and used as a template in the subsequent PCR that was performed using a Corbett Rotorgene 6000 using SensiMix SYBR mix (Bioline) and normalized to 18S rRNA.

Perks K.L., Ferreira N., Ermer J.A., Rudler D.L., Richman T.R., Rossetti G., Matthews V.B., Ward N.C., Rackham O, & Filipovska A. (2020). Reduced mitochondrial translation prevents diet-induced metabolic dysfunction but not inflammation. Aging (Albany NY), 12(19), 19677-19700.

+ Open protocol

+ Expand

Quantifying Mitochondrial RNA Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

The abundance of mitochondrial RNAs and unprocessed transcripts was measured on RNA isolated from cells using the miRNeasy RNA extraction kit (Qiagen). Levels of mitochondrial transcripts were measured from RNA isolated from cells or purified mitochondria. cDNA was prepared using the QuantiTect Reverse Transcription Kit (Qiagen)
and used as a template in the subsequent PCR that was performed using a Corbett Rotorgene 6000 using SensiMix SYBR mix (Bioline) and normalised to 18S rRNA.

Duff R.M., Shearwood A.M., Ermer J., Rossetti G., Gooding R., Richman T.R., Balasubramaniam S., Thorburn D.R., Rackham O., Lamont P.J, & Filipovska A. (2015). A mutation in MT-TW causes a tRNA processing defect and reduced mitochondrial function in a family with Leigh syndrome. Mitochondrion, 25.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!