Anti lamin b1 12987 1 ap

Western blot analysis was performed as described previously (16 (link)). Briefly, cells were suspended in RIPA buffer (50 mM Tris–HCl (pH 8.0), 420 mM NaCl, 0.5% sodium deoxycholate, 0.1% Sodium dodecyl sulfate, 1% NP-40) and sonicated. The extract was incubated for 30 min on ice, and then incubated at 95°C for 5 min. The extract was loaded and run on SDS-PAGE gel as standard protocols. For histone proteins, intensity was analyzed by OdysseyR CLx Imagins System(LI-COR). Anti-lamin B1 (12987-1-AP, Proteintech), anti-lamin A/C (3A6-4C11, Diagenode), anti-H2Aub (D27C4), anti-Ring1B (monoclonal antibody kindly gifted from Dr Koseki, RIKEN), anti-Ring1A (#2820, CST) were used.

The following antibodies were used in this study: anti-PGAM1 (NBP1-49532; Novus Biologicals), anti-CtIP (sc-271339; Santa Cruz Biotechnology, Inc.), anti-Mre11 (ab214; Abcam), anti–H2AX-pS139 (9718; Cell Signaling Technology), anti–β-actin (60008-1-Ig; Proteintech), anti–Lamin B1 (12987-1-AP; Proteintech), anti-GAPDH (60004-1-Ig; Proteintech), anti-RPA32 (ab2175; Abcam), anti–RPA32-pS4S8 (NBP1-23017; Novus Biologicals), anti-BrdU (5292; Cell Signaling Technology), anti-Cdh1 (ab3242; Abcam), anti-p21 (2947; Cell Signaling Technology), anti-RAD51 (sc-8349; Santa Cruz Biotechnology, Inc.), anti-PGD (sc-398977; Santa Cruz Biotechnology, Inc.), anti-PHGDH (ab57030; Abcam), anti-IgG (2729; Cell Signaling Technology), anti-Histone H3 (4620; Cell Signaling Technology), anti-p53 (9282; Cell Signaling Technology), anti-p73 (ab202474; Abcam), and anti–Cleaved Caspase-3 (9661; Cell Signaling Technology).

Erastin (HY-15763), ferrosatatin-1 (HY-100579), N-acetylcystein (HY–B0215) and MitoSOX Red (HY-D1055) were purchased from MedChem Express (Monmouth Junction, NJ). All-trans retinoic acid (ATRA, R2625), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, M2003) and 4′,6-diamidino-2-phenylindole (DAPI, D9542) were from Merck KGaA (Darmstadt, Germany). Anti-CYTL1 (ab129767) antibody was purchased from Abcam (Cambridge, UK). Anti-CSE (12217-1-AP), anti-ATG5 (10181-2-AP), anti-Keap1 (10503-2-AP), anti-Lamin B1 (12987-1-AP) and anti-p53 (10442-1-AP) antibodies were from Proteintech (Chicago, IL). Anti-HA (3724), anti-LC3B (3868), anti-Myc (2276) and anti-SQSTM1/p62 (8025) antibodies were from Cell Signaling Technology (Beverly, MA). Anti-GAPDH (sc-47724), anti-β-tubulin (sc-5274), anti-β-actin (sc-47778), anti-CBS (sc-133154), anti-Nrf2 (sc-365949), and anti-GPX4 (sc-166570) antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Lipofectamine 3000 (L3000), Lipofectamine RNAi MAX (13778), the lysosome-specific dye LysoTraker Red (L7528), and Alexa Fluor 488 goat anti-rabbit IgG (R37116) were purchased from Thermo Fisher Scientific (Waltham, MA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).