Wh0010413m1

Manufactured by Merck Group

The WH0010413M1 is a laboratory equipment product manufactured by Merck Group. It is designed to perform specific tasks in a laboratory setting. The core function of this product is to [insert concise, factual description of the product's core function without interpretation or extrapolation].

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Lab products found in correlation

Ab205270, by Abcam (2 mentions) Ab16048, by Abcam (2 mentions) A5316, by Merck Group (1 mentions) Ecl detection kit, by Bio-Rad (1 mentions) Bs 2090r, by Bioss Antibodies (1 mentions) Quantity one software, by Bio-Rad (1 mentions) Ab177487, by Abcam (1 mentions) Confocal microscopy, by Zeiss (1 mentions) Ab5076, by Abcam (1 mentions) Mab360, by Merck Group (1 mentions) Ab9260, by Merck Group (1 mentions) Ab15580, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Dojindo Laboratories (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Real envision hrp rabbit mouse, by Agilent Technologies (1 mentions) Permafluor, by Thermo Fisher Scientific (1 mentions)

3 protocols using wh0010413m1

Immunostaining Protocols for Cells and Tissue Sections

Cited 1 time

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For cultured cells, immunostaining was performed as previously described (X. Xu et al., 2020 (link)). Primary antibodies included rabbit anti‐YAP (ab205270, Abcam, 1:200), mouse anti‐YAP (WH0010413M1, Sigma, 1:200), mouse anti‐GFAP (MAB360, Millipore, 1:500), and rabbit anti‐Lamin B1 (ab16048, Abcam, 1:200).
For staining of tissue sections, immunostaining was conducted as previously described (C. Xie et al., 2020 (link)). Primary antibodies included rabbit anti‐YAP (ab205270, Abcam, 1:200), mouse anti‐YAP (WH0010413M1, Sigma, 1:200), mouse anti‐GFAP (MAB360, Millipore, 1:500), mouse anti‐NeuN (ab177487, Abcam, 1:500), goat anti‐Iba1 (ab5076, Abcam, 1:500), rabbit anti‐Lamin B1 (ab16048, Abcam, 1:200), and rabbit anti‐Ki67 (AB9260, Millipore, 1:200). Mounting was done after another three washes. Images were acquired by using a fluorescence microscopy or a confocal microscopy (Zeiss) and analyzed by Image J software.

Xu X., Shen X., Wang J., Feng W., Wang M., Miao X., Wu Q., Wu L., Wang X., Ma Y., Wu S., Bao X., Wang W., Wang Y, & Huang Z. (2021). YAP prevents premature senescence of astrocytes and cognitive decline of Alzheimer's disease through regulating CDK6 signaling. Aging Cell, 20(9), e13465.

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Comprehensive Western Blotting Protocol

Cited 1 time

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Western blotting was carried out as described previously (X. Xu et al., 2020 (link)). Primary antibodies used in this study including rabbit anti‐YAP (ab205270, Abcam, 1: 1, 000), mouse anti‐YAP (WH0010413M1, Sigma, 1:1, 000), rabbit anti‐p‐YAP (#13008, CST, 1:1, 000), rabbit anti‐LATS1 (#3477, CST, 1:1, 000), rabbit anti‐p‐LATS1 (ser909) (#9157, CST, 1:1, 000), rabbit anti‐MST1 (#3682, CST, 1:1, 000), rabbit anti‐p‐MST1/2 (Thr183/Thr180) (#49332, CST, 1:1, 000), rabbit anti‐SAV1 (#13301, CST, 1:1, 000), rabbit anti‐p‐MOB1 (Thr35) (#8699, CST, 1:1, 000), rabbit anti‐p53 (bs‐2090R, Bioss, 1:1, 000), rabbit anti‐Lamin B1 (ab16048, Abcam, 1: 1, 000), mouse anti‐CDK6 (#3136T, CST, 1:1, 000), rabbit anti‐FOXM1 (AV39518, Sigma, 1: 1, 000), and mouse anti‐PHD1 (F5303, Sigma, 1: 1, 000). Mouse anti‐β‐actin (A5316, Sigma, 1:10, 000) or rabbit anti‐GAPDH (#2118, CST, 1:5, 000) was used as a loading control. The protein signals were detected using ECL detection kit (Bio‐Rad) and analyzed using Quantity One software (Bio‐Rad).

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Immunohistochemical Analysis of Tongue Tissues

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Mice tongue tissues were fixed in 4% PFA, embedded in paraffin, and sectioned (4 μm) using standard procedures. IHC or IF staining was performed using an indirect method using primary antibodies recognizing YAP1 (WH0010413M1; Sigma), Ki67 (ab15580; Abcam), or TP63 (4A4; Abcam). Primary antibodies were detected using REAL EnVision HRP-rabbit/mouse (Dako) or Alexa Fluor 568 (Molecular Probes). Some slides were counterstained with Mayer’s hematoxylin (Muto) or 4′,6-diamidino-2-phenylindole (DAPI; Dojindo) before mounting using PermaFluor (Thermo Scientific). For Ki67 positivity studies, 200 cells per mouse were examined.

Omori H., Nishio M., Masuda M., Miyachi Y., Ueda F., Nakano T., Sato K., Mimori K., Taguchi K., Hikasa H., Nishina H., Tashiro H., Kiyono T., Mak T.W., Nakao K., Nakagawa T., Maehama T, & Suzuki A. (2020). YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma. Science Advances, 6(12), eaay3324.

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