Mouse anti bα clone 2g9

Manufactured by Merck Group

The Mouse anti-Bα clone 2G9 is a laboratory reagent used in research applications. It is an antibody that specifically binds to the Bα protein, a subunit of the 26S proteasome, a large protein complex involved in the degradation of unwanted or damaged proteins within cells. This antibody can be used to detect and study the Bα protein in various experimental settings, such as immunoblotting, immunoprecipitation, and immunohistochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti ha clone c29f4, by Cell Signaling Technology (2 mentions) Rabbit anti fyn clone epr5500, by Merck Group (2 mentions) Mouse anti ha clone 16b12, by Fortrea (2 mentions) Anti transferrin receptor clone h68, by Thermo Fisher Scientific (1 mentions) Anti actin clone c4, by Merck Group (1 mentions) Mouse anti p44 42 mapk erk1 2 l34f12, by Cell Signaling Technology (1 mentions) Mouse anti gsk3β clone 3d10, by Cell Signaling Technology (1 mentions) Rabbit anti phospho sfk y416 clone 100f9, by Cell Signaling Technology (1 mentions) Rabbit anti gfp clone d5, by Cell Signaling Technology (1 mentions) Rabbit anti phospho gsk 3β ser9, by Cell Signaling Technology (1 mentions) Clone c4, by Merck Group (1 mentions)

2 protocols using mouse anti bα clone 2g9

Antibody Characterization for Protein Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Unless indicated, all chemicals and drugs were from Sigma-Aldrich/Merck Millipore. Primary antibodies used in this study included the following: Rabbit anti-HA clone C29F4, anti-pSrc Tyr416 clones 100F9 and D49G4, anti-Na⁺/K⁺ ATPase #3010, and anti-GFP clone D5.1 (Cell Signaling Technology); rabbit Anti-APP clone Y188 (Abcam); mouse anti-HA clone 16B12 (Covance); rabbit anti-actin #AAN01 (Cytoskeleton Inc); anti-transferrin receptor clone H68.4 (Thermo Fisher Scientific); rabbit anti-methylated Lysine (Enzo Life Sciences); mouse anti-Fyn clone 25 #610163, anti-flotillin-1 clone 18, and anti-PP2Ac_α clone 46 (BD Transduction Laboratories); rabbit anti-Fyn clone EPR5500, mouse anti-Bα clone 2G9, anti-actin clone C4, anti-LCMT1 clone 4A4, anti-APP clone 22C11 (MAB348), and anti-demethyl PP2Ac clone 1D6 (Merck Millipore).

Taleski G., Schuhmacher D., Su H., Sontag J.M, & Sontag E. (2021). Disturbances in PP2A methylation and one-carbon metabolism compromise Fyn distribution, neuritogenesis, and APP regulation. The Journal of Biological Chemistry, 296, 100237.

+ Open protocol

+ Expand

Antibody-Based Analysis of Protein Signaling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Unless indicated, all chemicals were purchased from Sigma/Merck Millipore. Antibodies used in this study included mouse anti-HA (clone 16B12, Covance); rabbit anti-HA (clone C29F4, Cell Signaling Technology); rabbit anti-GFP (clone D5.1, Cell Signaling Technology); mouse anti-Bα (clone 2G9, Merck Millipore); mouse anti-PP2Ac_α/β (BD Transduction); goat anti-pY307 PP2Ac (Santa Cruz Biotechnology, Inc; denoted SC); rabbit anti-phospho-SFK (Y416) (clone 100F9, Cell Signaling Technology); rabbit anti-phospho-p44/42 MAPK (clone D13.14.4E, Cell Signaling Technology); mouse anti-p44/42 MAPK (ERK1/2) (L34F12, Cell Signaling Technology); rabbit anti-phospho-GSK-3β (Ser 9) (Cell Signaling Technology) and mouse anti-GSK3β (clone 3D10, Cell Signaling Technology); rabbit anti-Src (clone 32G6, Cell Signaling Technology); mouse anti-Src (clone GD11, Merck Millipore); rabbit anti-Fyn (clone EPR5500, Merck Millipore); mouse anti-Fyn (BD Transduction and Cell Signaling Technology); mouse and rabbit anti-p-Tyr (P-Tyr-100 and P-Tyr-1000, Cell Signaling Technology); rabbit anti-Tau (rPeptide), mouse anti-pSer202 Tau (CP13) (59 (link)) (a gift from Peter Davies). Mouse (clone C4, Merck Millipore) or rabbit (Cytoskeleton) anti-actin, and mouse (clone 236-10501, Life Technologies Australia Pty Ltd) or rabbit (Epitomics) anti-α-tubulin antibodies were used for protein normalization.

Sontag J.M., Schuhmacher D., Taleski G., Jordan A., Khan S., Hoffman A., Gomez R.J., Mazalouskas M.D., Hanks S.K., Spiller B.W., Sontag E, & Wadzinski B.E. (2022). A new paradigm for regulation of protein phosphatase 2A function via Src and Fyn kinase–mediated tyrosine phosphorylation. The Journal of Biological Chemistry, 298(8), 102248.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!