Anti rabbit igg horseradish peroxidase hrp conjugated secondary antibody

CG was dissolved in 0.05% dimethyl sulfoxide (DMSO) and used for biological assays. CellTiter 96® AQueous One Solution Cell Proliferation Assay Reagent [MTS; 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] was purchased from Promega (Madison, WI, USA), and propidium iodide (PI) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies specific to PARP, caspase-3, caspase-8, caspase-9, Bcl-2, Bcl-xL, Bax, Bid, and cytochrome c were sourced from Cell Signaling Technology (Beverly, MA, USA). Anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibody and anti-mouse IgG HRP-conjugated secondary antibody were obtained from Millipore (Billerica, MA, USA). Antibodies specific to p21, p27, cyclin D1, cyclin E, cyclin A, SOD-2, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl benzimidazoly carbocyanine chloride) was obtained from Enzo (New York, USA), FITC-annexin V apoptosis detection kit I was obtained from BD Biosciences (San Diego, CA, USA), and 2′,7′-dichlorofluorescin diacetate (DCF-DA) was procured from Abcam (Cambridge, UK).

CellTiter 96 AQ_ueous One Solution Cell Proliferation Assay Reagent [MTS, 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] was purchased from Promega (Madison, WI, USA). Propidium iodide (PI) and 4′,6-diamidino-2-phenylindole (DAPI) stain were purchased from Sigma-Aldrich (St. Louis, MO, USA). NE-PER Nuclear and Cytoplasmic Extraction Reagents were purchased from Pierce (Rockford, IL, USA). Antibodies specific to PARP, caspase-3, caspase-8, p53, Bcl-2, Bcl-xL, Bax, Bid, pRb, p-pRb, and cytochrome C were purchased from Cell Signaling Technology (Beverly, MA, USA). The anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibody and anti-mouse IgG HRP-conjugated secondary antibody were purchased from Millipore (Billerica, MA, USA). Antibodies specific to p27, p21, and glyceraldehyde 3-phospahte dehydrogenase (GAPDH) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl benzimidazolycarbocyanine chloride) was purchased from Enzo (Farmingdale, NY, USA). General-caspase inhibitor Z-VAD-fmk and caspase-8 inhibitor Z-IETD-fmk were purchased from R&D systems (Minneapolis, MN, USA). The FITC-Annexin V Apoptosis Detection Kit I was purchased from BD Biosciences (San Jose, CA, USA).