1 14c oleic acid

Manufactured by Cytiva

Sourced in United States

[1-14C] oleic acid is a radioactive isotope-labeled fatty acid product used in research applications. It is a key tool for studying lipid metabolism and related biological processes.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Mevinolin, by Merck Group (1 mentions) Atorvastatin, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Hepg2, by American Type Culture Collection (1 mentions) Tlc plate, by Macherey-Nagel (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Camphene, by Merck Group (1 mentions) L 35s methionine, by Cytiva (1 mentions) Enhanced chemiluminescence detection reagent, by Cytiva (1 mentions) L methyl 3h carnitine, by Cytiva (1 mentions)

2 protocols using 1 14c oleic acid

Cholesterol Biosynthesis Inhibition Assay

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Camphene, mevinolin, atorvastatin and U18666A were from Sigma-Aldrich. [2-¹⁴C] acetic acid sodium salt (250 μCi) and [1-¹⁴C] oleic acid (50 μCi) were purchased from Amersham Biosciences. ACAT inhibitor, F1394, was kindly provided by Dr Constantinos G. Panousis. Reagents for protein determination were from Bio-Rad. The human hepatic cell line, HepG2, was purchased from ATCC. Cell culture medium, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotics, penicillin and streptomycin, were from Gibco BRL Life Technologies. Lipoprotein-deficient serum (LPDS) was obtained from Autogen Bioclear UK Ltd. TLC plates were from Macherey Nagel.

Vallianou I, & Hadzopoulou-Cladaras M. (2016). Camphene, a Plant Derived Monoterpene, Exerts Its Hypolipidemic Action by Affecting SREBP-1 and MTP Expression. PLoS ONE, 11(1), e0147117.

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Radioactive Labeling Protocol

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[1-¹⁴C] oleic acid, L-[methyl-³H] carnitine, L-[³⁵S] methionine, and enhanced chemiluminescence detection reagents were bought from Amersham Pharmacia Biotech (Piscataway, NJ, USA), the BCA reagent was from Pierce (Rockford, IL, USA). The ABI DyeDeoxy Terminator Cycle Sequencing Kit was obtained from PE-Applied Biosystems (Foster City, CA, USA).

Yao M., Cai M., Yao D., Xu X., Yang R., Li Y., Zhang Y., Kido H, & Yao D. (2015). Abbreviated Half-Lives and Impaired Fuel Utilization in Carnitine Palmitoyltransferase II Variant Fibroblasts. PLoS ONE, 10(3), e0119936.

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