Polystyrene tubes

Manufactured by Sarstedt

Sourced in Germany

Polystyrene tubes are a type of laboratory equipment used for various applications. These tubes are made of sturdy polystyrene material and provide a reliable container for storing, transporting, and processing samples or reagents in a laboratory setting.

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Lab products found in correlation

Polybrene, by Merck Group (1 mentions) Facscanto 2, by BD (1 mentions) Densicheck plus, by bioMérieux (1 mentions) Vitek gp, by bioMérieux (1 mentions)

Close spelling variants of this product

Polystyrene FACS tubes

5 protocols using polystyrene tubes

Bovine Oocyte Maturation under Heat Stress

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Unless otherwise noted, reagents and chemicals were procured from MilliporeSigma (St. Louis, MO, USA). Oocytes were collected from abattoir-derived bovine ovaries as described previously
[3 (link)]. Oocyte maturation medium (OMM) and HEPES-TALP were prepared as described by Rispoli et al. [26 (link)]. Cumulus-oocyte complexes (COCs) were placed into OMM in polystyrene tubes (Sarstedt AG and Co., Nümbrecht, Germany) and matured at 38.5°C or at an elevated temperature
observed in heat-stressed cows [1 , 27 (link),28 (link),29 (link),30 (link),31 (link)]. Care was taken to ensure that only COCs with similarly sized cumulus cell vestments were
selected.

CAMPEN K.A., ABBOTT C.R., RISPOLI L.A., PAYTON R.R., SAXTON A.M, & EDWARDS J.L. (2018). Heat stress impairs gap junction communication and cumulus function of bovine oocytes. The Journal of Reproduction and Development, 64(5), 385-392.

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Effective Transduction of MSCs and Islet Coculture

Cited 1 time

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Viral supernatants (20 μl) were added to 200 000 MSC cultured in 25 cm² flasks in MSC medium supplemented with 8 μg/ml Polybrene (Sigma-Aldrich Inc, Saint Louis, MO, USA). Cells were incubated for 24 h at 37 °C, 5 % CO₂ and the media was replaced the following day. Transduction efficiency was analyzed for GFP expression using FACScanto II (BD Biosciences, San Diego, CA).
Human islets of Langerhans were manually picked and used one to three days post islet isolation. Islets were incubated with blue cell tracker (1-3 uM Cell Tracker, Molecular Probes, Eugene, OR) in 5cm Sterilin dishes (Sterilin Ltd, New Port, UK) in islet culture medium (see above), 1 h, 37 °C, followed by change of medium and 1h incubation in 37 °C. For creation of composite MSC-islets, we followed earlier established protocols for coating of the islets [13 , 37 (link)]. In short, approximately 200 islets were added to 5 mL polystyrene tubes (Sarstedt, Numbrecht, Germany) together with 185 000 GFP/luciferase-transduced MSC followed by careful mixing every 30 min during 2 h at room temperature (RT). Islets and composite MSC-islets were thereafter cultured in 5 cm Sterilin dishes with MSC complete medium (see above), 37 °C, overnight.

Fransson M., Brännström J., Duprez I., Essand M., Le Blanc K., Korsgren O, & Magnusson P.U. (2015). Mesenchymal stromal cells support endothelial cell interactions in an intramuscular islet transplantation model. Regenerative Medicine Research, 3, 1.

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In Vitro Oocyte Maturation Protocol

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Reagents and chemicals were obtained from MilliporeSigma (St. Louis, MO, USA) unless indicated otherwise. Oocytes were collected from abattoir-derived ovaries (Lawrence et al., 2004 (link)) located in Gaffney, South Carolina, USA (Brown Packing Co., Inc). Media were prepared per Rispoli et al. (2011) (link). Folltropin-V (FSH) was obtained from Vetrepharm Canada, INC. (London, ON, Canada); same batch was used throughout. Cumulus-oocyte complexes with compact cumulus cell vestments and homogenous ooplasm underwent in vitro maturation (Study 1: ~30 COCs per 0.5 ml maturation medium in polystyrene tubes; Sarstedt AG and Co., Nümbrecht, Germany; Study 2: 29 to 45 COCs (mean = 34.3 ± 0.66) per 0.5 mL in 4-well Nunc culture dishes; Thermo Fisher Scientific, Waltham, MA, USA). Incubator temperatures were verified before and during different studies using mercury thermometers sealed in media-filled bottles.

Rowinski J.R., Rispoli L.A., Payton R.R., Schneider L.G., Schrick F.N., McLean K.J, & Edwards J.L. (2021). Impact of an acute heat shock during in vitro maturation on interleukin 6 and its associated receptor component transcripts in bovine cumulus-oocyte complexes. Animal Reproduction, 17(4), e20200221.

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Bacterial Identification Using Vitek System

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Polystyrene tubes (12 mm × 75 mm) (Sarstedt) were filled with 3 mL of 0.45% sterile saline (CareFusion). Homogenous suspensions of bacteria were prepared in the 3 mL 0.45% sterile saline. Suspensions were examined using the DensiCheck Plus (BioMérieux) device to ensure that the suspensions were within the turbidity range of 0.5–0.63 (McFarland standard) for both Gram‐positive and Gram‐negative bacteria. The appropriate Vitek cards (Vitek GP and Vitek GN) (BioMérieux), which contained 64 wells with miniaturized selective media and biochemical reagents, were used for bacterial identification. The Vitek system identified the bacteria by filling the Vitek wells with the standard turbidity bacteria suspension in 3 mL of 0.45% sterile saline. The colour codes from the miniaturized selective media as well as the results of the miniaturized biochemical tests were analysed by the Vitek 2 Compact system software (Version 08.01, BioMérieux) to identify the bacteria species.

Adade E., Tawiah P.O., Roos C., Chuma I.S., Lubinza C.C., Mfinanga S.G., Knauf S, & Sylverken A.A. (2023). Antimicrobial susceptibility profile of oral and rectal microbiota of non‐human primate species in Ghana: A threat to human health. Veterinary Medicine and Science, 10(2), e1271.

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Breastfeeding Dynamics: Longitudinal Milk and Saliva Sampling

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Breastfeeding registration and self-report questionnaires regarding breastfeeding were collected through a mobile application on days 2, 5, 14, 30, and 60 postpartum or until breastfeeding cessation. Exclusive breastfeeding was defined as providing only human milk to the infant whereas partial breastfeeding involved providing a combination of human milk and infant formula.
The mothers provided five 3 mL samples of milk from one breast (not specified which) and three 2 mL samples of saliva on days 2, 5, 14, 30, and 60 postpartum or until breastfeeding cessation. Milk was collected by the mothers in their homes, in the morning, using a conventional manual breast pump provided by the research project. Women were not required to provide complete breast expressions, so milk was generally foremilk. Saliva samples were collected via passive drooling, where mothers allowed saliva to drip naturally into the tubes. Polypropene cryotubes (Sarstedt AG & Co. KG, Nümbrecht, Germany) were used for the saliva, and polystyrene tubes (Sarstedt AG & Co. KG, Nümbrecht, Germany) were used for the milk. Milk and saliva were frozen immediately at home and kept in home freezers (-20 • C) for up to 60 days, when they were transferred to a -80 • C freezer at the hospital.

Ericson J., McGuire M.K., Svärd A, & Hårdstedt M. (2024). Total Nitrite and Nitrate Concentration in Human Milk and Saliva during the First 60 Days Postpartum-A Pilot Study. Biomedicines, 12(6).

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