For reverse western blot, human cytokine antibody array kits (R&D Systems) were used, according to the manufacturer’s instructions. The pixel densities were analyzed using ImageJ for the experimental analysis and the Array Quick Spots Tool from HLImage++ (Western Vision Software) for the spot intensity analysis.
Human cytokine antibody array kits
Manufactured by R&D Systems
The Human Cytokine Antibody Array Kits from R&D Systems are multiplex assays designed to detect the relative levels of multiple human cytokines, chemokines, and other soluble proteins in a single sample. The kits use antibody-coated membranes to capture and detect the target analytes.
Automatically generated - may contain errors
2 protocols using human cytokine antibody array kits
1
Cytokine Antibody Array Analysis
2
Cytokine Signaling Pathway Analysis
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For reverse western blot, a human cytokine antibody array kits (R&D Systems) were used, according to the manufacturer's instructions. The western blotting analysis was performed with anti-STAT3, anti-Phospho STAT3, anti-CXCR2 (Cell Signaling, Danvers, MA), and anti-Actin antibody (Sigma, St. Louis, MO) as previously described [46 (link)].
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