Fluorescent mIHC was performed in serial sections of FFPE tumor tissue from each PDAC patient using the Opal 7-Colour Manual IHC Kit (PerkinElmer Hopkinton, Massachusetts, USA) according to the manufacturer’s protocol and as described in our previous article. In short, sections experienced primary antibodies including Rabbit monoclonal antibodies anti-CD68 (1:500, Abcam, Cambridge, MA, USA), anti-CXCR2 (1:500, Abcam), anti-CD206 (1000, Abcam), anti-CXCL1 (1:200, Abcam), anti-CXCL5 (1:200, Abcam), anti-CXCL8 (1:500, Abcam) and anti-CD8 (1:2000, Abcam), followed by HRP-conjugated secondary antibody and fluorescent dyes (Opal520, Opal570, Opal620, Opal690, and DAPI). Stained sections were scanned and processed by a Vectra PolarisTM Automated Quantitative Pathology Imaging System (AKOYA Biosciences, PerkinElmer, Massachusetts, USA).
Vectra polaristm automated quantitative pathology imaging system
Manufactured by Akoya Biosciences
Sourced in United States
The Vectra Polaris Automated Quantitative Pathology Imaging System is a laboratory equipment product developed by Akoya Biosciences. The core function of this system is to perform automated, high-throughput imaging and quantitative analysis of tissue samples.
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Lab products found in correlation
Anti cxcr2, by Abcam (1 mentions)
Anti cd206, by Abcam (1 mentions)
Anti cd8, by Abcam (1 mentions)
Opal 7 colour manual ihc kit, by PerkinElmer (1 mentions)
Anti cxcl5, by Abcam (1 mentions)
Anti cxcl8, by Abcam (1 mentions)
Anti cxcl1, by Abcam (1 mentions)
Anti cd68, by Abcam (1 mentions)
Ab14917, by Abcam (1 mentions)
Ab28364, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
2 protocols using vectra polaristm automated quantitative pathology imaging system
1
Multiplex Immunohistochemistry of Pancreatic Cancer
2
Multicolor Immunofluorescence Imaging Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Paraffin-embedded tissues were sliced into 4 µm sections and processed with a PDOne 6-color kit (Panovue, Beijing, China) according to the manufacturer’s instructions. Slices were subjected to heat-induced antigen retrieval, blocked with 10% goat serum containing TBST, and incubated with primary antibodies. After washing with phosphate-buffered saline containing 0.1% Tween-20, the slices were incubated with HRP-conjugated secondary antibodies, washed and incubated with TSA dyes. After nucleic acid staining with DAPI (Beyotime), the sliced sections were imaged with a VectraⓇ PolarisTM Automated Quantitative Pathology Imaging System (Akoya Biosciences). The antibodies used included anti-FOXP3 (1:100, 98377S, CST). anti-PD-1 (1:100, 43248, CST), anti-CD177 (1:2000, EPR22813–205, Abcam), and anti-CD31 (1:50, Ab28364, Abcam), anti-LYVE1 (1:20000, Ab14917, Abcam). Images quantification and distance analysis was conducted by HALO image analysis platform 3.6.4134 software (Indica Labs, NM, USA).
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