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Itaq universal sybr green supermix and bradford protein assay

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ITaq Universal SYBR Green Supermix is a ready-to-use solution for real-time PCR amplification and detection using the SYBR Green I dye. Bradford Protein Assay is a colorimetric protein assay for the quantification of total protein concentration in a sample.

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Lab products found in correlation

X tremegene 9 transfection reagent, by Roche (4 mentions) Rneasy plus mini kit, by Qiagen (4 mentions) Irdye secondary antibody, by LI COR (3 mentions) T7 rna polymerase, by New England Biolabs (2 mentions) Primary antibodies for eif3b and horseradish peroxidase hrp labeled secondary antibodies, by Santa Cruz Biotechnology (2 mentions) 14c labeled aa mixture, by MP Biomedicals (2 mentions) Trizol and dmem, by Thermo Fisher Scientific (2 mentions) Mtsea biotin xx, by Thermo Fisher Scientific (2 mentions) Dual luciferase reporter assay, by Promega (1 mentions) Phusion dna polymerase, by New England Biolabs (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Eif4g, by Cell Signaling Technology (1 mentions) Heat inactivated, by Merck Group (1 mentions) Dual luciferase assay, by Promega (1 mentions) Eif4e, by Cell Signaling Technology (1 mentions) Ncbp1, by Cell Signaling Technology (1 mentions) Phospho eif2α ser51, by Cell Signaling Technology (1 mentions) Larp1, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)

4 protocols using itaq universal sybr green supermix and bradford protein assay

1

Comprehensive RNA and Protein Analysis

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DMEM and TRIzol Reagent from Life Technologies; heat-inactivated FBS from Sigma Aldrich; T4 DNA Ligase I, polynucleotide kinase, proteinase K, Protoscript II reverse transcriptase, Phusion DNA polymerase, Vaccinia Capping System, T7 RNA polymerase from New England Biolabs; iTaq Universal SYBR Green Supermix and Bradford Protein Assay from Bio-rad; RNeasy Plus Mini Kit from Qiagen; DNA and RNA Clean and Concentrator 5 kits from Zymo Research; Endura Electrocompetent cells from Lucigen; Mighty Mix T4 DNA ligase from Takara; Dual luciferase reporter assay from Promega; and XtremeGENE 9 transfection reagent from Roche.
van den Elzen A.M., Watson M.J, & Thoreen C.C. (2022). mRNA 5′ terminal sequences drive 200-fold differences in expression through effects on synthesis, translation and decay. PLOS Genetics, 18(11), e1010532.
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2

Evaluating Cellular Stress Response

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Reagents were obtained from the following sources: antibodies for ATF4, S6K, phospho-T389-S6K, eIF2α, phospho-Ser51-eIF2α, 4E-BP1, GCN2, and UPF1 from Cell Signaling Technology; primary antibodies for eIF3b and horseradish peroxidase (HRP)-labeled secondary antibodies from Santa Cruz Biotechnology; IRDye secondary antibodies from LI-COR Biosciences; 14C-labeled AA mixture (011014750) from MP Biomedicals; Trizol and DMEM from Life Technologies; heat-inactivated fetal bovine serum (FBS), recombinant 4E-BP1, and 7mGDP from Sigma-Aldrich; RNase If, polynucleotide kinase, Proto-script II reverse transcriptase, and streptavidin-coated magnetic beads from New England Biolabs; iTaq Universal SYBR Green Supermix and Bradford Protein Assay from Bio-Rad; MTSEA biotin-XX from Pierce/Thermo Fisher Scientific; RNeasy Plus Mini Kit from QIAGEN; and XtremeGENE 9 transfection reagent from Roche.
Park Y., Reyna-Neyra A., Philippe L, & Thoreen C.C. (2017). mTORC1 Balances Cellular Amino Acid Supply with Demand for Protein Synthesis through Post-transcriptional Control of ATF4. Cell reports, 19(6), 1083-1090.
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3

Comprehensive protein expression analysis

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Reagents were obtained from the following sources: antibodies for S6K, phospho-T389-S6K, eIF2α, phospho-Ser51-eIF2α, Raptor, mTOR, 4EBP1, LARP1, NCBP1, eIF4E, eIF4G and PABP from Cell Signaling Technology; primary antibodies for eIF3b and HRP-labeled secondary antibodies from Santa Cruz Biotechnology; IRDye secondary antibodies from LI-COR; Dulbecco’s modified Eagle’s medium (DMEM) from Life Technologies; heat-inactivated Fetal Bovine Serum (IFS) and 7mGDP from Sigma Aldrich; DNase I, T4 DNA ligase 1, T4 RNA Ligase I, T7 RNA polymerase, polynucleotide kinase, Protoscript II reverse transcriptase, Vaccinia Capping System, Oligo d(T)25 Magnetic beads and streptavidin-coated magnetic beads from New England Biolabs; iTaq Universal SYBR Green Supermix and Bradford Protein Assay from Bio-rad; RNeasy Plus Mini Kit from Qiagen; Dual Luciferase Assay from Promega; and X-tremeGENE 9 transfection reagent from Roche.
Philippe L., Vasseur J.J., Debart F, & Thoreen C.C. (2017). La-related protein 1 (LARP1) repression of TOP mRNA translation is mediated through its cap-binding domain and controlled by an adjacent regulatory region. Nucleic Acids Research, 46(3), 1457-1469.
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4

Evaluating Cellular Stress Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents were obtained from the following sources: antibodies for ATF4, S6K, phospho-T389-S6K, eIF2α, phospho-Ser51-eIF2α, 4E-BP1, GCN2, and UPF1 from Cell Signaling Technology; primary antibodies for eIF3b and horseradish peroxidase (HRP)-labeled secondary antibodies from Santa Cruz Biotechnology; IRDye secondary antibodies from LI-COR Biosciences; 14C-labeled AA mixture (011014750) from MP Biomedicals; Trizol and DMEM from Life Technologies; heat-inactivated fetal bovine serum (FBS), recombinant 4E-BP1, and 7mGDP from Sigma-Aldrich; RNase If, polynucleotide kinase, Proto-script II reverse transcriptase, and streptavidin-coated magnetic beads from New England Biolabs; iTaq Universal SYBR Green Supermix and Bradford Protein Assay from Bio-Rad; MTSEA biotin-XX from Pierce/Thermo Fisher Scientific; RNeasy Plus Mini Kit from QIAGEN; and XtremeGENE 9 transfection reagent from Roche.
Park Y., Reyna-Neyra A., Philippe L, & Thoreen C.C. (2017). mTORC1 Balances Cellular Amino Acid Supply with Demand for Protein Synthesis through Post-transcriptional Control of ATF4. Cell reports, 19(6), 1083-1090.
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