Brain sections that included midline thalamus were incubated for 48 hours at room temperature in rabbit calretinin (CR) primary antibody (1:2000, RPCA-Calret; Encore Bio, FL) OR mouse calbindin (CB) primary antibody (1:500, MCA-5A9; Encore Bio, FL) in 5mL of 0.1% BSA. After this period, tissue was washed 3 times for 5 minutes in 0.1 M PB and placed in goat anti-rabbit OR anti-mouse biotinylated secondary antibodies respectively (1:500, BP-9200/9100; Vector Labs, CA) for 6 hours. After PB washes, the tissue was reacted in a solution containing avidin–biotin complex (Vectastain Elite ABC Kit, PK-6100; Vector Laboratories, CA) for one hour at room temperature, followed by three 5-minute rinses in 0.1 M PB. The peroxidase reaction was produced by incubating the sections for 5 to 12 minutes in a DAB substrate solution (SK-4100; Vector Labs, CA). Reacted tissue was then mounted on gelatin coated slides, dehydrated in methanol and xylene before being cover-slipped with Permount or Vectashield antifade mounting medium with DAPI (H-1900, Vector Labs, CA).
Bp 9200 9100
Manufactured by Vector Laboratories
The BP-9200/9100 is a high-precision blood pressure monitoring device designed for laboratory and research applications. It provides reliable and accurate measurements of systolic and diastolic blood pressure, as well as heart rate. The device is equipped with advanced sensors and algorithms to ensure consistent and reproducible results.
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2 protocols using bp 9200 9100
1
Immunohistochemical Labeling of Brain Regions
2
Immunohistochemical Staining of Brain Regions
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Brain sections that included midline thalamus were incubated for 48 hours at room temperature in rabbit calretinin (CR) primary antibody (1:2000, RPCA-Calret; Encore Bio, FL) OR mouse calbindin (CB) primary antibody (1:500, MCA-5A9; Encore Bio, FL) in 5mL of 0.1% BSA. After this period, tissue was washed 3 times for 5 minutes in 0.1 M PB and placed in goat anti-rabbit OR anti-mouse biotinylated secondary antibodies respectively (1:500, BP-9200/9100; Vector Labs, CA) for 6 hours. After PB washes, the tissue was reacted in a solution containing avidin-biotin complex (Vectastain Elite ABC Kit, PK-6100;
Vector Laboratories, CA) for one hour at room temperature, followed by three 5-minute rinses in 0.1 M PB. The peroxidase reaction was produced by incubating the sections for 5 to 12 minutes in a DAB substrate solution (SK-4100; Vector Labs, CA). Reacted tissue was then mounted on gelatin coated slides, dehydrated in methanol and xylene before being cover-slipped with Permount or Vectashield antifade mounting medium with DAPI (H-1900, Vector Labs, CA) .
Vector Laboratories, CA) for one hour at room temperature, followed by three 5-minute rinses in 0.1 M PB. The peroxidase reaction was produced by incubating the sections for 5 to 12 minutes in a DAB substrate solution (SK-4100; Vector Labs, CA). Reacted tissue was then mounted on gelatin coated slides, dehydrated in methanol and xylene before being cover-slipped with Permount or Vectashield antifade mounting medium with DAPI (H-1900, Vector Labs, CA) .
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