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Truseq stranded messenger rna protocol

Manufactured by Illumina
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The TruSeq stranded messenger RNA (mRNA) protocol is a laboratory technique used for the preparation and sequencing of mRNA samples. The protocol involves the isolation, purification, and conversion of mRNA into a sequencing-ready library, which can then be used for downstream analysis, such as gene expression profiling or transcript identification.

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Lab products found in correlation

Nanodrop one spectrophotometer, by Thermo Fisher Scientific (1 mentions) High output 75 cycles kit, by Illumina (1 mentions) Fragment analyzer system, by Agilent Technologies (1 mentions) Mirneasy mini kit, by Qiagen (1 mentions) Nextseq 500 platform, by Illumina (1 mentions)

Close spelling variants of this product

TruSeq Stranded RNA protocol TruSeq Stranded protocol TruSeq RNA protocol TruSeq protocol

2 protocols using truseq stranded messenger rna protocol

1

Optimized RNA-seq Pipeline for GBM Subtyping

Cited 2 times
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We used the Illumina TruSeq stranded messenger RNA protocol and sequenced 100 million reads. Reads were aligned using STAR25 (link) and Gencode genes were quantified using featureCounts.26 (link) Ninety-five percent of reads mapped the reference genome. We normalized the counts with DESeq2 and adjusted the quantification to account for GC bias27 (link) and batch effects28 (link) between The Cancer Genome Atlas (TCGA) GBM RNA-seq and our sample. The normalized expression data are used to identify GBM subtypes.29 (link)
Wrzeszczynski K.O., Frank M.O., Koyama T., Rhrissorrakrai K., Robine N., Utro F., Emde A.K., Chen B.J., Arora K., Shah M., Vacic V., Norel R., Bilal E., Bergmann E.A., Moore Vogel J.L., Bruce J.N., Lassman A.B., Canoll P., Grommes C., Harvey S., Parida L., Michelini V.V., Zody M.C., Jobanputra V., Royyuru A.K, & Darnell R.B. (2017). Comparing sequencing assays and human-machine analyses in actionable genomics for glioblastoma. Neurology: Genetics, 3(4), e164.
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2

RNA Extraction and Sequencing of Endometrial Samples

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Total RNA was extracted from the thawed endometrial biopsy samples using the miRNeasy Mini Kit (ref. 1038703; Qiagen, Hilden, Germany) according to RNeasy FFPE Handbook protocol instructions (Qiagen, Hilden, Germany). The amount of total RNA isolated from each tissue was quantified using the NanoDrop One spectrophotometer (ThermoFisher, Vilnius, Lithuania), and the integrity of the extracted RNA was assessed using the Fragment Analyzer System (Agilent, Santa Clara, CA). Nine endometrial samples from each group (9 from the COVID-19 group and 9 from the control group) with the highest DV200 values were selected for RNA-seq analysis. Sequencing libraries were constructed using the TruSeq stranded messenger RNA protocol (Illumina, San Diego, CA), with poly-A selection for ribosomal messenger RNA depletion, followed by chemical fragmentation and complementary DNA synthesis, as per the manufacturer’s instructions. The libraries were sequenced in 2 runs using the NextSeq 500 platform (Illumina, San Diego, CA) at a single-end read length of 75 nucleotides using the High Output 75 cycles kit (Illumina, San Diego, CA).
de Miguel-Gómez L., Sebastián-León P., Romeu M., Pellicer N., Faus A., Pellicer A., Díaz-Gimeno P, & Cervelló I. (2022). Endometrial gene expression differences in women with coronavirus disease 2019. Fertility and Sterility, 118(6), 1159-1169.
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