Maxwell 16 blood dna kit

Manufactured by Promega

Sourced in Germany

The Maxwell 16 Blood DNA Kit is a lab equipment product designed for the automated purification of genomic DNA from whole blood samples. It provides a streamlined and standardized process for DNA extraction, enabling efficient and reliable sample preparation for downstream applications.

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Lab products found in correlation

Maxwell rsc instrument, by Promega (1 mentions) Maxwell rsc buccal swab kit, by Promega (1 mentions) Cfx96 real time thermal cycler, by Bio-Rad (1 mentions) Quantstudio real time pcr software, by Thermo Fisher Scientific (1 mentions) Cfx maestro software, by Bio-Rad (1 mentions)

Close spelling variants of this product

Maxwell 16 (79 citations) Blood DNA kit (4 citations) Maxwell kits (2 citations) Maxwell 16 Tissue and Blood DNA purification kits (2 citations)

5 protocols using maxwell 16 blood dna kit

Blood DNA Extraction and Genotyping

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DNA was extracted from blood samples collected in 6 ml K₃EDTA tubes (Vacuette; Cruinn Diagnostics, Ireland) using the Maxwell 16 Blood DNA kit (Promega, Madison, WI, USA) as per manufacturer’s instructions. Extracted DNA samples were analysed for quality and quantity using a Nanodrop spectrophotometer and normalised to 50 ng/µL for genotyping analysis. Genotyping was performed at Weatherby’s Scientific Ltd. (Johnstown, Naas, Co. Kildare, Ireland) using the IDBv3 chip which contains 50,855 markers³⁶.

Johnston D., Mukiibi R., Waters S.M., McGee M., Surlis C., McClure J.C., McClure M.C., Todd C.G, & Earley B. (2020). Genome wide association study of passive immunity and disease traits in beef-suckler and dairy calves on Irish farms. Scientific Reports, 10, 18998.

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Specificity Evaluation of SARS-CoV-2 RT-LAMP Assay

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To determine the specificity of the SARS-CoV-2 RT-LAMP assay, SARS-CoV Frankfurt 1 RNA [#004N-02005, European Virus Archive (EVAg)] [13 (link)] and nucleic acid extracts from 20 different samples from respiratory pathogens (Respiratory Verification Panel, #NATRVP-QIA, ZeptoMetrix Corporation, Buffalo, USA) were tested. Nucleic acid was extracted from Respiratory Verification Panel samples utilising a Maxwell 16 extraction robot together with the Maxwell 16 Blood DNA Kit (#AS1400, Promega GmbH, Mannheim, Germany).

Mautner L., Baillie C.K., Herold H.M., Volkwein W., Guertler P., Eberle U., Ackermann N., Sing A., Pavlovic M., Goerlich O., Busch U., Wassill L., Huber I, & Baiker A. (2020). Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP). Virology Journal, 17, 160.

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Automated DNA Extraction from Blood and Buccal Samples

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DNA was extracted from EDTA‐stabilized whole blood or buccal swap using the Maxwell 16 Blood DNA Kit or the Maxwell RSC Buccal Swab Kit on the Maxwell RSC instrument (Promega, US).

Kløve‐Mogensen K., Steffensen R., Masmas T.N., Glenthøj A., Haunstrup T.M., Ratcliffe P., Höglund P., Hasle H, & Nielsen K.R. (2022). ABO, secretor, and Lewis carbohydrate histo‐blood groups are associated with autoimmune neutropenia of early childhood in Danish patients. Transfusion, 62(8), 1636-1642.

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Peripheral Blood DNA Extraction

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DNA was extracted from peripheral blood using the Maxwell16 Blood DNA Kit on the Maxwell16 Instrument (Promega Corporation).

Nielsen K.R., Bojsen S.R., Masmas T.N., Fjordside A., Baech J., Haunstrup T.M, & Steffensen R. (2021). Association between human leukocyte antigens (HLAs) and human neutrophil antigens (HNAs) and autoimmune neutropenia of infancy in Danish patients. Pediatric Allergy and Immunology, 32(4), 756-761.

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Evaluation of Commercial RT-qPCR Kits for SARS-CoV-2 Detection

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Several commercially available RT-qPCR kits based on fluorescently labelled hydrolysis probes are routinely used to test for SARS-CoV-2 at the Bavarian Health and Food Safety Authority (LGL). In this study, the commercial test kits ampliCube Coronavirus SARS-CoV-2 (#50143 (50144), Mikrogen Diagnostik, Neuried, Germany) and FTD SARS-CoV-2 (#FTD-114-96, Fast Track Diagnostics (A Siemens Healthineers Company), Esch-sur-Alzette, Luxembourg) were used following previous RNA extraction utilising a Maxwell 16 extraction robot together with the Maxwell 16 Blood DNA Kit (#AS1400, Promega GmbH, Mannheim, Germany). Reactions were performed in accordance to each manufacturer’s instructions, respectively, and carried out in a CFX96 real-time thermal cycler (Bio-Rad, Hercules, USA) or a Quantstudio 7 real-time thermal cycler (Thermo Fisher Scientific, Waltham, USA). CFX Maestro software (Bio-Rad, Hercules, USA; version 1.1) or QuantStudio™ Real-Time PCR software (Thermo Fisher Scientific, Waltham, USA) were used for data acquisition and analysis.

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