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2 protocols using furaltadone

1

Immunoaffinity-based detection of nitrofuran metabolites

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Furaltadone, furazolidone, nitrofurantoin, Furaltadone, AMOZ, AOZ, AHD, SEM, 2-NP-AMOZ, 2-NP-AOZ, 2-NP-AHD, 2-NP-SEM, 2-nitrobenzaldehyde, 4-carboxybenzaldehyle, and horseradish peroxidase-labeled goat anti-mouse IgG were purchased from Sigma-Aldrich (St. Louis, MO, USA). Goat anti-mouse IgG conjugated with 655 nm QDs was obtained from Invitrogen Corp (Carlsbad, CA, USA). N,N-dicyclohexylcarbodiimide (DCC), N,N-dimethylformamide (DMF), N-hydroxysuccinimide (NHS), dimethyl sulfoxide (DMSO), ethyl acetate, and all other chemicals were of analytical grade and purchased from Sinopharm Chemical Reagent Co. Ltd. (Beijing, China) unless indicated otherwise.
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2

Comprehensive Veterinary Drug Extraction

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All solvents (HPLC/analytical grade) were purchased from Fluka (Sigma- Aldrich, St. Louis, MO, USA). Trichloroacetic acid (TCA) crystals and the reagents to prepare an EDTA-McIlvaine buffer solution, pH 4 (disodium hydrogen phosphate dihydrate, citric acid monohydrate and EDTA) were purchased from Fluka.
Formic acid (98–100%) was obtained from Riedel-de Haën (Sigma-Aldrich, St. Louis, MO, USA).
The extraction cartridges (Oasis HLB 3 mL, 60 mg) were provided by Waters (Milford, MA, USA).Amoxicillin, ampicillin, benzylpenicillin, cefalexin, cefquinome, ceftiofur, chloramphenicol, chlortetracycline, ciprofloxacin, danofloxacin, dimetridazole, doxycycline, enrofloxacin, florfenicol, florfenicol amine, flumequine, furaltadone, furazolidone, lincomycin, lomefloxacin hydrochloride, marbofloxacin, nalidixic acid, nitrofurazone, oxolinic acid, oxytetracycline, ronidazole, spyramicin, sulphadiazine, sulphadimethoxine, sulphadimidine, sulphamerazine, sulphathiazole, tetracycline hydrochloride, thiamphenicol, tilmicosine, tinidazole, trimethoprim, tylosin, and enrofloxacin d5 as the internal standards (IS) (purity >98%) were used and purchased from Fluka (Sigma-Aldrich, St. Louis, MO, USA).
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