PEG, citric acid, maleic acid, dichloromethane (DCM), acryloyl chloride (AC), magnesium sulfate (MgSO4), ammonium persulfate (APS), tetramethylethylenediamine (TEMED), and 5-FU were purchased from Sigma Aldrich (St. Louis, MO). Dox-hydrochloride (Tocris Bioscience, Ellisville, MO), PLGA 50:50 (Lakeshore Biomaterials, Birmingham, AL), CMC (AK Scientific Inc., Union City, CA), potassium carbonate (K2CO3) (Alfa Aesar, Ward Hill, MA), and triethylamine (TEA, MP Biomedicals, Solon, OH) were purchased and used as obtained. Dulbecco’s Modified Eagle Media (DMEM) and trypsin-ethylenediaminetetraacetic acid (Trypsin-EDTA) were purchased from Invitrogen (Carlsbad, CA). Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Lawrenceville, GA). Adult human dermal fibroblast (HDFs), A431 epidermoid carcinoma cells and G361 human melanoma cells were purchased from ATCC (Rockville, MD).
Dox hydrochloride
Manufactured by Bio-Techne
Sourced in United States
Dox-hydrochloride is a chemical compound that is commonly used in laboratory settings. It serves as a fluorescent dye, which can be used to stain and visualize various biomolecules, such as proteins and nucleic acids. The core function of Dox-hydrochloride is to provide a means for researchers to detect and analyze these biological components within their experiments.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions)
Maleic acid, by Merck Group (1 mentions)
Acryloyl chloride, by Merck Group (1 mentions)
Dulbecco s modified eagle media dmem, by Thermo Fisher Scientific (1 mentions)
Trypsin ethylenediaminetetraacetic acid trypsin edta, by Thermo Fisher Scientific (1 mentions)
Potassium carbonate k2co3, by Thermo Fisher Scientific (1 mentions)
Citric acid, by Merck Group (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
Penicillin, by GE Healthcare (1 mentions)
Streptomycin, by GE Healthcare (1 mentions)
Trypan blue, by Corning (1 mentions)
Supersignal west femto maximum sensitivity substrate kit, by Thermo Fisher Scientific (1 mentions)
Tween 20, by Boston BioProducts (1 mentions)
Tris buffered saline (tbs), by Thermo Fisher Scientific (1 mentions)
Pe annexin 5 apoptosis detection kit, by BD (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Cell proliferation reagent wst 1, by Roche (1 mentions)
2 protocols using dox hydrochloride
1
Stimuli-responsive Drug Delivery System
2
Doxorubicin-Induced Apoptosis Assay
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The following reagents were used in this study: DMEM (Corning Incorporated, Corning, NY, USA); penicillin and streptomycin (10,000 U/mL each; GE Healthcare Life Sciences, Logan, UT, USA); FBS (Atlanta Biologicals, Lawrenceville, GA, USA); DOX hydrochloride (Tocris Bioscience, Bristol, UK); SuperSignal™ West Femto Maximum sensitivity substrate kit (Thermo Fisher Scientific, Waltham, MA, USA), Trypan blue (Corning Incorporated), cell proliferation reagent (WST-1) (Roche Diagnostic Corporation, Indianapolis, IN, USA), PE Annexin V Apoptosis Detection Kit (BD Biosciences, San Diego, CA, USA), Tris-buffered saline (Thermo Fisher Scientific), and Tween 20 (Boston Bioproducts, Ashland, MA, USA).
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