Mouse cd45 percp

Cell staining was performed at 4 °C in phosphate-buffered saline (PBS) supplemented with 1% FBS unless otherwise indicated. Antibodies and reagents used for flow cytometry and functional studies were as follows: mouse CD45 PerCP, mouse CD8a FITC, mouse CD4 PE, mouse MHCI FITC, mouse MHCII PE (eBioscience, San Diego, CA, USA). Data were collected using a CytoFLEX flow cytometer (Beckman Coulter) and analyzed using CytoExpert.

The aqueous extract of Salvia plebeia R. (FIE-SP, SPW) was provided by FromBio (Kyounggido, Korea). Briefly, dried leaves of SP were cultivated in Gyeongsangbuk-do, Korea, soaked with water, and the mixture was then heated for 8 h at 80–85 °C, filtered, and dried by a spray dryer. Dulbecco’s modified eagle medium (DMEM), RPMI-1640, fetal bovine serum (FBS), and penicillin were obtained from Gibco BRL (Grand Island, NY, USA). The CyQUANT™ lactate dehydrogenase (LDH) cytotoxicity assay kit and the immunoglobulin ELISA assay kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Concanavalin A, IL-1β, IL-2, IL-4, IL-10, IL-12, IFN-γ, and TNF-α ELISA assay kits were purchased from R&D system (Mckinley Place, NE, USA). Mouse CD45 PerCP, mouse CD8a FITC, mouse CD4 PE, mouse major histocompatibility complex (MHCI), FITC, and mouse MHCII PE antibody were purchased from eBioscience (San Diego, CA, USA).