HUVECs were seeded in culture dishes 24 h prior to the transfection. For enhanced or decreased expression of miR-126, miRNA mimics control (mimics NC), miR-126 mimics, miRNA inhibitor control (inhibitor NC), or miR-126 inhibitor were purchased from GenePharma (Suzhou, Jiangsu, China). miRNAs were transfected into HUVECs using Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. At 24 h, cells were harvested for subsequent analysis.
Mirna mimics control mimics nc
Manufactured by GenePharma
Sourced in China
The MiRNA mimics control (mimics NC) is a laboratory tool used in microRNA (miRNA) research. It serves as a negative control for miRNA mimics experiments, providing a baseline for comparison to assess the specific effects of miRNA mimics on target genes or cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Mirna inhibitor control inhibitor nc, by GenePharma (3 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (2 mentions)
Mir 126 mimic, by GenePharma (1 mentions)
Puromycin, by Merck Group (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Pcdna3.1 control, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Oe nc, by GenePharma (1 mentions)
Opti mem culture medium, by Thermo Fisher Scientific (1 mentions)
Mir 326 inhibitor, by GenePharma (1 mentions)
4 protocols using mirna mimics control mimics nc
1
MiR-126 Expression Regulation in HUVECs
2
Modulating miR-215 and RAD54B expression
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As described by Jiang et al in previous research.19 For enhanced or decreased expression of miR‐215, miRNA mimics control (mimics NC), miR‐215 mimics, miRNA inhibitor control (inhibitor NC), or miR‐215 inhibitor were purchased from GenePharma. miRNAs were transfected into cells using Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer's instructions. After 24 hours, cells were harvested for subsequent analysis.
The adenovirus vector constructed by UCBio (Changsha, China) was used for stable overexpression or knockdown of RAD54B in cells according to the manufacturer's instructions, and selected with puromycin (Sigma‐Aldrich) for 2‐3 weeks to obtain stable cell lines.
The adenovirus vector constructed by UCBio (Changsha, China) was used for stable overexpression or knockdown of RAD54B in cells according to the manufacturer's instructions, and selected with puromycin (Sigma‐Aldrich) for 2‐3 weeks to obtain stable cell lines.
3
Culturing and Transfecting Human VSMCs
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Human VSMCs were obtained from the Cell Center of the Chinese Academy of Medical Sciences (Shanghai, China), cultured in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS, HyClone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma, St. Louis, MO, USA) at 37°C in 5% CO2. The cells in the logarithmic growth phase were used for follow-up experiments.
We purchased pcDNA3.1-control, pcDNA3.1-FGF1, miRNA mimics control (mimics-NC), miRNA inhibitors control (inhibitors-NC), miR-188-3p mimics, and miR-188-3p inhibitors from GenePharma Co., Ltd. (Shanghai, China). VSMCs were harvested and seeded into a 24-well plate at a density of 3×105 cells/well, and cell transfection was performed after 24 h of culture. VSMCs were transfected using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) conforming to the protocols.
We purchased pcDNA3.1-control, pcDNA3.1-FGF1, miRNA mimics control (mimics-NC), miRNA inhibitors control (inhibitors-NC), miR-188-3p mimics, and miR-188-3p inhibitors from GenePharma Co., Ltd. (Shanghai, China). VSMCs were harvested and seeded into a 24-well plate at a density of 3×105 cells/well, and cell transfection was performed after 24 h of culture. VSMCs were transfected using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) conforming to the protocols.
4
Modulating miR-326 and HDAC3 in BMSCs
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BMSCs were transfected with miR-326 mimics (5′-CCCCCGTCCCGGAAACACTT-3′) for overexpression the miR-326 level, miRNA mimics control (mimics NC), miR-326 inhibitor (5′-TTACAAAGGCCCTGCCCTGCCCCC-3′) for knockdown the miR-326 level, and miRNA inhibitor control (inhibitor NC) (GenePharma, China). HDAC3 overexpression plasmid pcDNA3.1-HDAC3 (HDAC3 OE) and its negative control plasmid (NC OE) purchased from GenePharma were used to transfect chondrocytes. Cell transfections were performed using the transfection reagent Lipofectamine 2000 (Invitrogen, USA) according to manufacturer's instructions. In brief, cells (3 × 105) were seeded into 6-well plates for cell transfection upon cell fusion of 60~80%. 500 μL Opti-MEM culture medium (Gibco, USA) containing 5 μL Lipofectamine 2000 and 5 μL miR-326 mimics, inhibitors, or controls was added to each well and cultured in an incubator at 37°C for 6 h. The captured cells were treated with 100 nM Trichostatin A or 50 μmol/L AG490 at room temperature for 4 h.
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