Reverace

For qRT-PCR assays, total RNAs were extracted from various tissues, panicles and seeds at different developmental stages using TRIzol reagent (Invitrogen), and reverse transcribed using oligo(dT) primer and ReverAce (TOYOBO). cDNA was synthesized from 2 μg of total RNA treated with DnaseI (TOYOBO) and used as templates for RT-PCR or qRT-PCR. Rice actin gene was used for normalization. Single and double asterisks represent significant difference determined by the Student’s t-test at ^∗P < 0.05 and ^∗∗P < 0.01 respectively.
For OsRA2 expression analysis, the roots, leaves, sheaths, culms, shoot apical meristem (SAM) were collected from around 30-day-old plants; panicles meristems less than 1 cm were collected under microscope, and flowers were collected from heading stage.

Total RNAs were extracted from the leaves or young panicles of the plants using the TRIzol (Invitrogen), and then reverse transcribed using ReverAce (TOYOBO). cDNA was synthesized from 2 μg of total RNA treated with DNase I (TOYOBO) and used as template.