All three parts of the SI (duodenum, jejunum, ileum) and livers were fixed in 4% neutral-buffered formalin (Carl Roth GmbH, Karlsruhe, Germany) for 24–48 h and stored in 30% sucrose until cryosectioning. Intestinal and hepatic sections of 7 μm were cut (HM 560 Cryo-Star; Microm International GmbH, Walldorf, Germany) and stained with Oil Red O (ORO) (Sigma-Aldrich, St. Louis, MO) and Mayer’s hematoxylin (Carl Roth) to visualize neutral lipids and nuclei, respectively. Microscopic images were taken in 20-40× magnification using an Olympus BX63 microscope (Olympus, Shinjuku, Japan) equipped with a DP73 camera.
Neutral buffered formalin
Manufactured by Carl Roth
Sourced in Germany
4% neutral-buffered formalin is a fixative solution primarily used in the preservation and preparation of biological samples for microscopic examination. It contains 4% formaldehyde in a buffered aqueous solution, maintaining a neutral pH. The solution is designed to preserve the structural integrity of tissues and cells without altering their morphological characteristics.
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Lab products found in correlation
3 protocols using neutral buffered formalin
1
Lipid Visualization in Intestinal and Hepatic Tissues
2
Histopathological Evaluation of SAV-Induced Lesions
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Heart and pancreas were excised and placed in 4% neutral buffered formalin (Carl Roth, Karlsruhe, Germany). Sections for histopathology were processed by standard paraffin wax techniques and stained with hematoxylin and eosin (H&E). Tissue sections were examined as a blind study and the presence or absence of lesions recorded. A scoring system was used to evaluate the severity of SAV induced lesions in heart and exocrine pancreas [21 (link)].
3
Lipid Visualization in Intestinal and Hepatic Tissues
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