Tissue microarrays (TMAs) were constructed with 5-µm sections of tissue punches from 20 FFPE paired samples (at baseline and at 3 weeks of TH treatment) from patients included in the study. Each sample was examined for pathological validation by two independent investigators who were blinded to the patient’s status before and after the TMA was constructed. Samples were stained using an automated tissue immunostainer (Dako, Canada). Slides were probed to validate the expression of the proteins of interest identified in the proteomic profiling assay with selective antibodies (Supplementary Table S1 ) and were visualized under a light microscope.
Automated tissue immunostainer
Manufactured by Agilent Technologies
Sourced in Canada
The Automated Tissue Immunostainer is a laboratory instrument designed for the automated processing and staining of tissue samples. It performs critical tasks such as antigen retrieval, primary antibody incubation, and detection, enabling efficient and consistent sample preparation for immunohistochemical analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using automated tissue immunostainer
1
Tissue Microarray Analysis of Protein Expression
2
Immunohistochemical Analysis of Ovarian Carcinoma
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TMAs were acquired from the Pan-canadian platform for the development of biomarker-driven subtype specific management of ovarian carcinoma (COEUR study). Slides were deparaffinized in citrate buffer containing 0.05 % Tween at 97 °C for 20 min, washed with PBS and incubated with 3 % peroxide. After treatment, slides were submerged in a citrate buffer (0.01 M citric acid, pH 6.0) for 15 min, and incubated with a protein blocking serum-free reagent (Dako Canada). The TMAs were stained by an immunoperoxidase method using an automated tissue immunostainer (Dako Canada) with DABchromogen. The TMAs were counter stained with hematoxilin and slides were scanned using HAMAMATSU Nanozoomer (Boston, MA) and each tissue section were scored according to the staining intensity where negative staining = 0, low staining = 1+, moderate staining = 2+ and high staining = 3+. Two separate TMAs containing different section of the same tumor were scored and the mean intensity was used.
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