Hla a24 02

Manufactured by STEMCELL

The HLA-A24:02 is a laboratory product used for research purposes. It is a recombinant protein that represents the HLA-A24:02 allele. The HLA-A24:02 allele is a common human leukocyte antigen (HLA) class I molecule. This product can be used in various immunological and cell biology studies.

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Lab products found in correlation

2 protocols using hla a24 02

Humanized Mouse Model for Gastric Cancer

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Details of the humanized mouse model experiment have been described previously (108 ). One- to three-day-old NSG pups were sublethally irradiated at 1 Gy and engrafted with 1 × 10⁵ human CD34⁺ cord blood cells (HLA-A24:02; STEMCELL Technologies) via intrahepatic injection. Mice with more than 10% human immune-cell reconstitution (calculated based on the proportion of human CD45 relative to the sum of human and mouse CD45) were included in the study. In total, two diffuse-type gastric cancer cell lines of HLA-A24:02 subtype were selected for the experiment (KLF2 positive, GSU; KLF2 negative, SNU1750; refs. 109, 110 ). For each cell line, five humanized mice and five NSG mice were injected with the tumor cells and observed for one month. Mice were sacrificed at the end of one month, necropsies were performed, and tumors were harvested and sequenced (bulk RNA-seq).

Kumar V., Ramnarayanan K., Sundar R., Padmanabhan N., Srivastava S., Koiwa M., Yasuda T., Koh V., Huang K.K., Tay S.T., Ho S.W., Tan A.L., Ishimoto T., Kim G., Shabbir A., Chen Q., Zhang B., Xu S., Lam K.P., Lum H.Y., Teh M., Yong W.P., So J.B, & Tan P. (2022). Single-Cell Atlas of Lineage States, Tumor Microenvironment, and Subtype-Specific Expression Programs in Gastric Cancer. Cancer Discovery, 12(3), 670-691.

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Humanized Mouse Model for Tumor Engraftment

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One to three-day-old NOD-scid Il2rγ^null (NSG) pups were sublethally irradiated at 1 Gy and engrafted with 1×10⁵ human CD34⁺ cord blood cells (HLA-A24:02, Stemcell Technologies) via intrahepatic injection. Mice with more than 10% human immune cell reconstitution (calculated based on the proportion of human CD45 relative to the sum of human and mouse CD45) were included in the study. In total, five cell lines of HLA-A24:02 subtype were selected for the experiment (two APB_high (SNU1750 and GSU), one APB_int (YCC21) and two APB_low (NCC 59 and SNU16) cell lines).
For each cell-line, five humanised mice and five NSG mice were injected with the tumour cells and observed for 1 month. Mice were sacrificed at the end of 1 month; necropsies was performed; and tumours were harvested for further analysis (online supplemental methods).

Sundar R., Huang K.K., Kumar V., Ramnarayanan K., Demircioglu D., Her Z., Ong X., Bin Adam Isa Z.F., Xing M., Tan A.L., Tai D.W., Choo S.P., Zhai W., Lim J.Q., Das Thakur M., Molinero L., Cha E., Fasso M., Niger M., Pietrantonio F., Lee J., Jeyasekharan A.D., Qamra A., Patnala R., Fabritius A., De Simone M., Yeong J., Ng C.C., Rha S.Y., Narita Y., Muro K., Guo Y.A., Skanderup A.J., So J.B., Yong W.P., Chen Q., Göke J, & Tan P. (2021). Epigenetic promoter alterations in GI tumour immune-editing and resistance to immune checkpoint inhibition. Gut, 71(7), 1277-1288.

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