L 540

Classic Hodgkin lymphoma cell lines (L428, L540, and L591) were purchased from the German Collection of Microorganisms and Cell Cultures. Another cHL cell line, AM‐HLH was kindly gifted from Dr. Ohno H at Tenri Institute of Medical Research.¹⁰ Among cHL cell lines, L591 and AM‐HLH are infected with EBV. A T cell line, Jurkat, was obtained from the Japanese Cancer Research Resources Bank. We used two LCLs from two donors, LCL2 and LCL3, which had previously been established from PBMCs purchased from Cellular Technology Limited by the method based on the past report.¹¹ CHO cell lines stably expressing CD30L and its control without CD30L expression were described previously.¹² L540 and L591 were cultured in RPMI‐1640 (Sigma‐Aldrich) with 20% FBS. AM‐HLH was cultured in RPMI‐1640 with 13% FBS. CHO cells were cultured in Ham's F‐12 (Fujifilm Wako Pure Chemical) medium with 10% FBS. Other cell lines were cultured in RPMI‐1640 with 10% FBS.

HL cell lines (L428, KMH2, HDLM2, and L540) were purchased from the German Collection of Microorganisms and Cell Cultures. B‐cell lines (Namalwa, BJAB, and Ramos) and a T‐cell line (Jurkat) were purchased from the Japanese Cancer Research Resources Bank and RIKEN BioResource Research Center, respectively. Cells were cultured in RPMI 1640 supplemented with 10% or 20% fetal bovine serum (FBS) and antibiotics.