Human chromogranin a elisa kit

Salivary cortisol and chromogranin A were obtained from saliva samples. The athletes were requested to refrain from ingesting stimulating (e.g., coffee and chocolate) or dye containing substances, and from brushing their teeth during the three hours before assessment. Saliva samples were collected by chewing regular cotton salivette sampling devices (Sarstedt, Nümbrecht, Germany), thus without chemical stimulants. Samples were kept on ice and then stored at -20°C until the day of analysis. At the day of the analysis, saliva samples were centrifuged 10 min at 2000 x g to remove particulate material. Hormonal determinations were obtained by using the Human Chromogranin A ELISA Kit (MyBiosource, San Diego, CA, USA) and Human Cortisol ELISA Kit (Diagnostics Biochem Canada Inc, London, Ontario, Canada) according to the manufacturers’ directions and were expressed as ng/ml. All samples were processed in duplicate during the same assay section.

Salivary cortisol, DHEA, and chromogranin A were obtained from stimulated whole saliva samples. Human salivary cortisol was measured using radioenzyme immunoassay (Diagnostics Biochem Canada Inc.) using a commercial enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions. We used an ELISA kit (MyBiosource) to measure the DHEA hormone and the Human Chromogranin A ELISA Kit (MyBiosource) to measure the chromogranin A levels. The saliva samples were kept on ice and stored at -20°C until analysis. On the day of analysis, saliva samples were centrifuged for 10 min at 2000 × g to remove particulate material. All samples were processed in triplicate during the same assay. Statistical analyses were conducted using the average of the three repeated measurements.