Cd164

Manufactured by BioLegend

Sourced in United States

CD164 is a cell surface glycoprotein that functions as a sialomucin. It is expressed on various cell types, including hematopoietic stem and progenitor cells, endothelial cells, and some epithelial cells. CD164 plays a role in cell-cell and cell-matrix interactions, but its precise biological functions are still under investigation.

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Lab products found in correlation

Cd146, by BioLegend (5 mentions) Facsaria 2, by BD (2 mentions) Magnetic activated cell sorting (macs), by Miltenyi Biotec (2 mentions) Histopaque, by Merck Group (2 mentions) Cd202b tie 2, by BioLegend (2 mentions) Cd90 thy1, by BioLegend (2 mentions) Facscanto 2, by BD (2 mentions) Podoplanin, by Thermo Fisher Scientific (1 mentions) Igg1 pe, by BD (1 mentions) Igg2a apc, by BioLegend (1 mentions) Fitc igg1, by BioLegend (1 mentions) Version 10, by FlowJo (1 mentions) Cd105, by BioLegend (1 mentions) Igg1 apc, by BioLegend (1 mentions) Perm wash buffer, by BD (1 mentions) Cd11b, by BD (1 mentions) Cd150, by BioLegend (1 mentions)

Spelling variants of this product

Mouse anti-CD164 antibody (67D2) (2 citations)

5 protocols using cd164

Isolation and Characterization of Skeletal Cells

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Femoral heads were obtained from young males, elderly males, premenopausal and postmenopausal females undergoing total hip arthroplasty. The bone marrow of the femoral head was isolated and human skeletal cells were separated from RBCs and bone dust by density gradient separation using 1:1 Histopaque 1.119 g/mL. The buffy coat was collected, washed with staining media, and the resulting cell suspension was depleted of CD45 + cells by magnetic-activated cell sorting (MACS). Cells were blocked with mouse IgG and stained with fluorochrome-conjugated antibodies against CD45 (Biolegend), CD235 (Biolegend), CD31(Invitrogen), CD202b (Biolegend), CD146 (Biolegend), Podoplanin (Invitrogen), CD164 (Biolegend), CD73 (Biolegend) at a dilution of 1:100. Flow cytometry was performed on FACS Aria II in the shared FACS Facility in the Lorry I. Lokey Stem Cell Institute. Gating schemes were established with fluorescence-minus-one (FMO) controls and 4’,6-diamidino-2-phenylindole (DAPI) was used for viability staining.

Andrew T.W., Koepke L.S., Wang Y., Lopez M., Steininger H., Struck D., Boyko T., Ambrosi T.H., Tong X., Sun Y., Gulati G.S., Murphy M.P., Marecic O., Telvin R., Schallmoser K., Strunk D., Seita J., Goodman S.B., Yang F., Longaker M.T., Yang G.P, & Chan C.K. (2022). Sexually dimorphic estrogen sensing in skeletal stem cells controls skeletal regeneration. Nature Communications, 13, 6491.

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Isolation and Phenotyping of Human Skeletal Cells

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Human skeletal cells were separated from red blood cells (RBCs) and bone dust by density gradient separation using 1:1 Histopaque of density 1.119 g/mL (Sigma-Aldrich, St Louis, MO, USA). The buffy coat was collected, washed with staining media, and the resulting cell suspension was depleted of CD45 + cells by magnetic-activated cell sorting (MACS) (Miltenyi, Cat#130–045-801). Cells were blocked with mouse IgG and stained with fluorochrome-conjugated antibodies against CD45 (BioLegend, Cat#304029-BL), CD235a (BioLegend, Cat#306612-BL), CD31 (Thermo Fisher Scientific™, Cat#13–0319-82), CD202b (Tie-2) (BioLegend, Cat#334204), CD146 (BioLegend, Cat#342010), PDPN (Thermo Fisher Scientific™, Cat#17–9381-42), CD90 (THY1; BioLegend, Cat#328110), CD164 (BioLegend, Cat#324808), and CD73 (BioLegend, Cat#344016). Flow cytometry was performed on FACS Aria II (BD Biosciences). Gating schemes were established with fluorescence-minus-one (FMO: staining with all fluorophores except one) controls and negative propidium iodide (PI) (Sigma-Aldrich, Cat#P4170) staining (1 μg/ml) was used as a measure for cell viability.

Tevlin R., Griffin M., Chen K., Januszyk M., Guardino N., Spielman A., Walters S., Gold G.E., Chan C.K., Gurtner G.C., Wan D.C, & Longaker M.T. (2023). Denervation during mandibular distraction osteogenesis results in impaired bone formation. Scientific Reports, 13, 2097.

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Chondrocyte Phenotypic Characterization

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The chondrocytes were fixed using a fixation/permeabilization solution kit (cat. no. 554715; BD Biosciences) and stained with CD34 (cat. no. 343607; BioLegend, Inc.; 1:100), CD44 (cat. no. 338807; BioLegend, Inc.; 1:100), CD59 (cat. no. 304711; BioLegend, Inc.; 1:100), CD74 (cat. no. 326811; BioLegend, Inc.; 1:100), CD90 (cat. no. 328109; BioLegend, Inc.; 1:100), CD105 (cat. no. 323205; BioLegend, Inc.; 1:100), CD146 (cat. no. 361015; BioLegend, Inc.; 1:100), CD164 (cat. no. 324805; BioLegend, Inc.; 1:100), SOX9-Alexa-647 (cat. no. 565493; BD Pharmingen; BD Biosciences; 1:200), ACAN-PE (cat. no. sc-33695; Santa Cruz Biotechnology, Inc.; 1:100), IgG1-Alexa-647 (cat. no. 557732; BD Pharmingen; BD Biosciences; 1:100), IgG1-PE (cat. no. 400112; BioLegend, Inc.; 1:100), IgG1-APC (cat. no. 400122; BioLegend, Inc.; 1:100), IgG1-FITC (cat. no. 400109; BioLegend, Inc.; 1:100) or IgG2a-APC (cat. no. 400221; BioLegend, Inc.; 1:100) for 30 min at 4˚C. The dilution of the antibody for FACS was ranged from 1:100-1:200. After staining, cells were washed with Perm/Wash Buffer (cat. no. 554715; BD Biosciences) and analyzed by flow cytometry (FACS Canto II; BD Biosciences). Data were analyzed using FlowJo version 10.7 software (FlowJo LLC).

Park J., Park H., Lee Y.L., Weon S., Kim Y.G., Yang J.H., Nam B., Jo S, & Kim T.H. (2021). Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritis. Experimental and Therapeutic Medicine, 22(2), 808.

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Isolation and Characterization of Human Skeletal Cells

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Human skeletal cells were separated from red blood cells (RBCs) and bone dust by density gradient separation using 1:1 Histopaque of density 1.119 g/mL (Sigma-Aldrich, Cat#11191). The buffy coat was collected, washed with staining media, and the resulting cell suspension was depleted of CD45+ cells by magnetic-activated cell sorting (MACS) (Miltenyi, Cat#130–045-801). Cells were blocked with mouse IgG and stained with fluorochrome-conjugated antibodies against CD45 (BioLegend, Cat#304029-BL), CD235a (BioLegend, Cat#306612-BL), CD31 (Thermo Fisher Scientific™, Cat#13–0319-82), CD202b (Tie-2) (BioLegend, Cat#334204), CD146 (BioLegend, Cat#342010), PDPN (Thermo Fisher Scientific™, Cat#17–9381-42), CD90 (THY1; BioLegend, Cat#328110), CD164 (BioLegend, Cat#324808), and CD73 (BioLegend, Cat#344016). Flow cytometry was performed on FACS Aria II (BD Biosciences). Gating schemes were established with fluorescence-minus-one (FMO: staining with all fluorophores except one) controls and negative propidium iodide (PI) (Sigma-Aldrich, Cat#P4170) staining (1 μg/ml) was used as a measure for cell viability.

Chan C.K., Gulati G.S., Sinha R., Tompkins J.V., Lopez M., Carter A.C., Ransom R.C., Reinisch A., Wearda T., Murphy M., Brewer R.E., Koepke L.S., Marecic O., Manjunath A., Seo E.Y., Leavitt T., Lu W.J., Nguyen A., Conley S.D., Salhotra A., Ambrosi T.H., Borrelli M.R., Siebel T., Chan K., Schallmoser K., Seita J., Sahoo D., Goodnough H., Bishop J., Gardner M., Majeti R., Wan D.C., Goodman S., Weissman I.L., Chang H.Y, & Longaker M.T. (2018). Identification of the Human Skeletal Stem Cell. Cell, 175(1), 43-56.e21.

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Characterization of Tendon and Muscle Cells

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The tendon and muscle cells were washed with Perm/Wash buffer (554715; BD Biosciences, San Jose, CA, USA) and stained with Alkaline phosphatase (ALP; 327308; BioLegend, San Diego, CA, USA), CD11b (550019; BD Pharmingen, San Diego, CA, USA), CD34 (343607; BioLegend), CD45 (368511; BioLegend), CD59 (3044711; BioLegend), CD74 (326811; BioLegend), CD90 (328109; BioLegend), CD150 (323205; BioLegend), CD146 (361015; BioLegend), or CD164 (324805; BioLegend) for 30 min at 4 °C. Following staining, the cells were fixed using a fixation/permeabilization solution kit (554715; BD Biosciences) and washed with Perm/Wash buffer (554715; BD Biosciences). Stained cells were analyzed by flow cytometry (FACS Canto II; BD Biosciences) and data images were managed with Flowjo software (BD Biosciences; San Jose, CA, USA).

Lee J.K., Jo S., Lee Y.L., Weon S., Song J.S., Sung I.H, & Kim T.H. (2021). Effect of Muscle Cell Preservation on Viability and Differentiation of Hamstring Tendon Graft In Vitro. Cells, 10(4), 740.

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