Both methanol and acetic acid were HLPC grade and bought from ROE Scientfic Inc. (Newark, U.S.A). All amino acids authentic compounds such as valine, histidine, arginine and lysine were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Creatine, L-carnitine (BR) and vitamin B6 (BR) were purchased from Shanghai Source Leaf Biological Technlogy Co., Ltd (Shanghai, China) with 98%, 98% and 99% purity, respectively. The deionized water used for experiments was provided by a Milli-Q water purification system (Billerica, USA). All standard solutions were prepared in deionized water and then used for ESI-MS analysis without further treatment.
Lysine
Manufactured by Sinopharm
Sourced in China
Lysine is a laboratory-grade amino acid used in various biochemical applications. It serves as a building block for proteins and is commonly employed in cell culture media formulations and other biotechnological processes.
Automatically generated - may contain errors
Lab products found in correlation
Valine, by Sinopharm (2 mentions)
Glucose, by Sinopharm (2 mentions)
Glycine, by Sinopharm (2 mentions)
Ascorbic acid, by Sinopharm (2 mentions)
Histidine, by Sinopharm (1 mentions)
Arginine, by Sinopharm (1 mentions)
Glutathione (gsh), by Sinopharm (1 mentions)
Acrylamide, by Sinopharm (1 mentions)
Ferrocene, by Sinopharm (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Cysteine, by Merck Group (1 mentions)
Homocysteine, by Merck Group (1 mentions)
Acetone, by Sinopharm (1 mentions)
Hydrogen peroxide, by Sinopharm (1 mentions)
Urea, by Sinopharm (1 mentions)
Ethylene glycol, by Sinopharm (1 mentions)
Rutin, by Sinopharm (1 mentions)
Cysteine, by Sinopharm (1 mentions)
Fructose, by Sinopharm (1 mentions)
Vertex 80v spectrometer, by Bruker (1 mentions)
6 protocols using lysine
1
Quantification of Metabolites by ESI-MS
2
Synthesis of Functional Materials
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Ethylene glycol (purity
> 99%), ferrocene (purity > 98%), glucose (purity > 98%),
hydrogen
peroxide (H2O2, 30% water solution), glutathione
(reduced, purity > 98%), acetone (purity > 99%), glycine (purity
>98%), lysine (purity > 98%), acrylamide (AM, purity > 98%),
and urea
(purity > 98%) were purchased from Sinopharm. BSA (purity >96%) was
purchased from Aladdin. CBIS (purity > 99%), glutathione (purity
>98%), cysteine (purity > 98%), homocysteine (purity > 90%),
BIS (purity
> 99%), and 2-hydroxy-4′-(2-hydroxyethoxy)-2-methyl-propiophe
(purity > 98%) were purchased from Aldrich. The chemicals were
used
directly without further purification.
> 99%), ferrocene (purity > 98%), glucose (purity > 98%),
hydrogen
peroxide (H2O2, 30% water solution), glutathione
(reduced, purity > 98%), acetone (purity > 99%), glycine (purity
>98%), lysine (purity > 98%), acrylamide (AM, purity > 98%),
and urea
(purity > 98%) were purchased from Sinopharm. BSA (purity >96%) was
purchased from Aladdin. CBIS (purity > 99%), glutathione (purity
>98%), cysteine (purity > 98%), homocysteine (purity > 90%),
BIS (purity
> 99%), and 2-hydroxy-4′-(2-hydroxyethoxy)-2-methyl-propiophe
(purity > 98%) were purchased from Aldrich. The chemicals were
used
directly without further purification.
3
Carboxylatopillar[5]arene Nanomaterial Synthesis
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Rhodamine 6G
(R6G), citric acid, ethylenediamine, N-hydroxysuccinimide
(NHS), and 1-ethyl-3-(3-(dimethylamino)propyl)carbodimide hydrochloride
(EDC·HCl) were purchased from Aladdin. NaOH was obtained from
Energy Chemical. Chlorine salts (Na+, K+, Ca2+, Mg2+), gallic acid, ascorbic acid, glucose,
fructose, lysine, cysteine, and rutin were bought from Sinopharm Chemical
Reagent Co., Ltd., China. Carboxylatopillar[5]arene (CP[5]) was synthesized
according to our published procedure.41 (link)Transmission electron microscopy (TEM) images were acquired
by using a JEM 2100F instrument operating at an accelerating voltage
of 200 kV. Fourier transform infrared (FTIR) spectra were obtained
by using a Bruker Vertex 80 V spectrometer. Powder X-ray diffraction
(PXRD) measurements were conducted by using a PANalytical B.V. Empyrean
powder diffractometer. Ultraviolet–visible (UV–vis)
spectra were recorded using a Shimadzu UV-2550 instrument. Thermogravimetric
analysis (TGA) was performed on a Q500 instrument. Fluorescence spectra
were recorded in quartz cuvettes by using a Shimadzu RF-5301PC spectrophotometer.
ζ potentials were recorded on a Particle Sizing Systems Z3000
instrument.
(R6G), citric acid, ethylenediamine, N-hydroxysuccinimide
(NHS), and 1-ethyl-3-(3-(dimethylamino)propyl)carbodimide hydrochloride
(EDC·HCl) were purchased from Aladdin. NaOH was obtained from
Energy Chemical. Chlorine salts (Na+, K+, Ca2+, Mg2+), gallic acid, ascorbic acid, glucose,
fructose, lysine, cysteine, and rutin were bought from Sinopharm Chemical
Reagent Co., Ltd., China. Carboxylatopillar[5]arene (CP[5]) was synthesized
according to our published procedure.41 (link)Transmission electron microscopy (TEM) images were acquired
by using a JEM 2100F instrument operating at an accelerating voltage
of 200 kV. Fourier transform infrared (FTIR) spectra were obtained
by using a Bruker Vertex 80 V spectrometer. Powder X-ray diffraction
(PXRD) measurements were conducted by using a PANalytical B.V. Empyrean
powder diffractometer. Ultraviolet–visible (UV–vis)
spectra were recorded using a Shimadzu UV-2550 instrument. Thermogravimetric
analysis (TGA) was performed on a Q500 instrument. Fluorescence spectra
were recorded in quartz cuvettes by using a Shimadzu RF-5301PC spectrophotometer.
ζ potentials were recorded on a Particle Sizing Systems Z3000
instrument.
4
HAMPT Combination Therapy Protocol
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Stock solution of HAMPT is composed of the following: aspirin 75 mg (Aladdin Reagent Company, China), lysine 200 mg (Sinopharm Chemical Reagent Co., China), mifepristone 10 mg (Shanghai New Hualian Co., China), and doxycycline hyclate 50 mg (Shanghai Civi Chemical Technology Co., China). Drug doses were chosen based on the human doses of the four drugs [36 (link), 51 , 53 (link)-54 (link)] and converted to mouse doses according to the body surface area index [24 (link)]. The concentrations of the four drugs used in the in vitro assay were chosen based on the same dose ratio of the four drugs composed and used in the animal studies. The combination was so formulated to target different pathways of cancer metastasis.
5
Electrochemical Biosensor Fabrication
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GlutaOx, glutamate monosodium
salt monohydrate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
(EDC), N-hydroxysuccinimide (NHS), Nafion solution
(5 wt%), bovine serum albumin (BSA), and gold(III) chloride trihydrate
(HAuCl4·3H2O) were purchased from Sigma.
GO–COOH and MWNT–COOH were purchased from Xianfeng Nanomaterials
Technology Co., Ltd. Chloroplatinic acid hexahydrate (H2PtCl6) was purchased from Macleans Biochemical Technology
Co., Ltd. (Shanghai, China). Ascorbic acid, lysine, valine, aspartic
acid, alanine, isoleucine, phenylalanine, leucine, glycine, and proline
were purchased from Sinopharm Chemical Reagent Co., Ltd. Company (Shanghai
China). The rest of the reagents are of analytical grade.
salt monohydrate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
(EDC), N-hydroxysuccinimide (NHS), Nafion solution
(5 wt%), bovine serum albumin (BSA), and gold(III) chloride trihydrate
(HAuCl4·3H2O) were purchased from Sigma.
GO–COOH and MWNT–COOH were purchased from Xianfeng Nanomaterials
Technology Co., Ltd. Chloroplatinic acid hexahydrate (H2PtCl6) was purchased from Macleans Biochemical Technology
Co., Ltd. (Shanghai, China). Ascorbic acid, lysine, valine, aspartic
acid, alanine, isoleucine, phenylalanine, leucine, glycine, and proline
were purchased from Sinopharm Chemical Reagent Co., Ltd. Company (Shanghai
China). The rest of the reagents are of analytical grade.
6
Fabrication of PEN-PVP Nanofiltration Membranes
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PEN ([η] = 1.15 dL g−1) synthesized in the laboratory as described in previous literature50 (link) was used as the base polymer in the casting solution. Reagent grade PVP-k30 supplied by Macklin Biochemical Technology Co. Ltd. was used as pore-forming agent. The chemical structure of PEN and PVP were shown in Fig. 1 . Reagent grade DMAc (≥99.8% purity) and NMP (≥99.5% purity) supplied by Macklin, were used as mixed solvent without further purification. SiO2 NPs (7–40 nm, hydrophilic-380 type) and 3-glycidyloxypropyl (dimethoxy) methylsilane (GPMS, KH560) were obtained from Aladdin Biochemical Technology Co. Ltd. Lysine, Hydrochloric acid, Ethanol were purchased from Sinopharm Group Chemical Reagent Co. Ltd. Bovine serum albumin (BSA) with molecular weight of 68 000 Da was obtained from Energy Chemical Co. Ltd. Deionized water purified with Millipore system (Millipore, France) was used as the coagulation bath.
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