1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-Dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE), 1,2-Dimyristoyl-sn-glycero-3-phospho-rac-glyceol (DMPG), 1,2-Dimyristoyl-sn-glycero-3-phospho-l -serine (DMPS) were purchased from Larodon (Solna, Sweden). The expression vectors pET21a and pET28a were purchased from Stratagene (La Jolla, CA, USA). Escherichia coli Shuffle T7 Express Competent cells were purchased from New England BioLabs (Beijing, China). IPTG (isopropyl β-d -1-thiogalactopyranoside) and ampicillin were obtained from Sangon Biotech, Shanghai Co., Ltd. (Shanghai, China). Ni2+-NTA Sepharose fast-flow and anion exchange chromatography (Q-Sepharose XL) columns were purchased from GE Healthcare (Boston, MA, USA). Bicinchoninic acid (BCA) Protein Assay Kit was from Sangon Biotech, Shanghai Co., Ltd. (Shanghai, China). All other regents were of analytical grade.
Escherichia coli shuffle t7 express competent cells
Manufactured by New England Biolabs
Sourced in China
Escherichia coli Shuffle T7 Express Competent cells are a strain of E. coli designed for the expression of recombinant proteins. They feature the ability to promote the formation of disulfide bonds, which can aid in the proper folding of certain proteins.
Automatically generated - may contain errors
2 protocols using escherichia coli shuffle t7 express competent cells
1
Protein Expression and Purification
2
Phospholipid Synthesis and Purification
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Phospholipids used in the present studies were all purchased from Larodan (Solna,
1,2-diarachidoyl-sn-glycero-3-phosphocholine (DAPC) (>99%) and used without further purification unless otherwise stated. Expression vectors of pFL-B62cl were from GeneCopoeia Inc. (USA). Escherichia coli SHuffle T7 Express Competent cells were purchased from New England BioLabs (Beijing, China). IPTG (isopropyl β-D-1-thiogalactopyranoside) and Ampicillin were from Sangon Biotech, Shanghai Co., Ltd. (Shanghai, China). All other reagents were of analytical grade.
1,2-diarachidoyl-sn-glycero-3-phosphocholine (DAPC) (>99%) and used without further purification unless otherwise stated. Expression vectors of pFL-B62cl were from GeneCopoeia Inc. (USA). Escherichia coli SHuffle T7 Express Competent cells were purchased from New England BioLabs (Beijing, China). IPTG (isopropyl β-D-1-thiogalactopyranoside) and Ampicillin were from Sangon Biotech, Shanghai Co., Ltd. (Shanghai, China). All other reagents were of analytical grade.
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