All animal studies were performed according to guidelines approved by the Investigational Animal Care and Use Committee of the National Institute for Viral Diseases Control and Prevention of the China CDC. We performed viral challenge by i.n. inoculation of 105 TCID50 of A/PR/8/34 (10-fold 50% lethal dose) to anesthetized 6- to 8-week adult female C57 BL/6 mice in 50 μL PBS. Intraperitoneal administration of 1,6-bis PC or/and peramivir (Abmole Bioscience, USA) was started 48 h after virus infection. A total of 4 types of treatment regimens (0.1 mM 1,6-bis PC, 0.02 mM 1,6-bis PC, 3 mg peramivir, and 3 mg peramivir plus 0.1 mM 1,6-bis PC per kg each day) were administered until day 7 postinfection. Compound 1,6-bis PC was synthesized in SKS Chem according to a previous report (Pepys et al., 2006 (link)). All compounds were diluted in TC (0.01 M Tris plus 0.14 M NaCl and 0.002 M CaCl2, pH 8.0) buffer. If the mice lost over 25% of their initial body weight, they were humanely euthanized and necropsied.
Peramivir
Manufactured by AbMole
Sourced in United States
Peramivir is a laboratory product that functions as a neuraminidase inhibitor. It is used in biological research and experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Tryple express, by Thermo Fisher Scientific (2 mentions)
Mycaway one step mycoplasma detection kit, by Yeasen (2 mentions)
Neuroeasy maintenance medium, by Cellapy (2 mentions)
Nucleozin, by Apexbio (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Essential 8 medium, by Thermo Fisher Scientific (2 mentions)
Brain derived neurotrophic factor (bdnf), by Thermo Fisher Scientific (2 mentions)
3 protocols using peramivir
1
Evaluating Antivirals against Influenza in Mice
2
Cell Culture and Treatment Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human HEK293T (CRL-11268) and Madin-Darby Canine Kidney (MDCK) cells (ATCC, CRL-2936) were maintained in Dulbecco's Minimal Essential Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 units/mL penicillin, and 100 µg/mL streptomycin (Invitrogen). Authentication and test for the free of mycoplasma were performed with MycAwayTM one-step mycoplasma detection kit (Yeasen). Astrocytes were purchased from Cellapy (CA2315106) and cultured in NeuroEasy maintenance medium (Cellapy). Human embryonic stem cells (hESCs) were obtained from Harvard Stem Cell Institute. hESCs were routinely checked for pluripotent, normal karyotype, mycoplasma free and cultured in feeder-free conditions on Matrigel-coated plates with Essential 8 medium (GIBCO) and passaged with TrypLETM express (GIBCO). 10 μM Peramivir (M3222, AbMole BioScience), 1 μM Nucleozin (A3670, Apexbio), 50 μM PYC-12, 20 μM RO3306, 10 μM WH-L50B, 20 ng/mL BDNF (450-02, PeproTech), 20 ng/mL GDNF (450-10, PeproTech), 20 ng/mL NT3 (450-03, PeproTech) were used in this study.
3
Cell Culture Protocols for Research
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Human HEK293T (CRL-11268) and Madin-Darby Canine Kidney (MDCK) cells (ATCC, CRL-2936) were maintained in Dulbecco's Minimal Essential Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 units/mL penicillin, and 100 µg/mL streptomycin (Invitrogen). Authentication and test for the free of mycoplasma were performed with MycAway TM one-step mycoplasma detection kit (Yeasen).
Astrocytes were purchased from Cellapy (CA2315106) and cultured in NeuroEasy maintenance medium (Cellapy). Human embryonic stem cells (hESCs) were obtained from Harvard Stem Cell Institute. hESCs were routinely checked for pluripotent, normal karyotype, mycoplasma free and cultured in feeder-free conditions on Matrigel-coated plates with Essential 8 medium (GIBCO) and passaged with TrypLE TM express (GIBCO). 10 µM Peramivir (M3222, AbMole BioScience), 1 µM Nucleozin (A3670, Apexbio), 50 µM PYC-12, 20 µM RO3306, 10 µM WHL-50B, 20 ng/mL BDNF (450-02, PeproTech), 20 ng/mL GDNF (450-10, PeproTech), 20 ng/mL NT3 (450-03, PeproTech) were used in this study.
Astrocytes were purchased from Cellapy (CA2315106) and cultured in NeuroEasy maintenance medium (Cellapy). Human embryonic stem cells (hESCs) were obtained from Harvard Stem Cell Institute. hESCs were routinely checked for pluripotent, normal karyotype, mycoplasma free and cultured in feeder-free conditions on Matrigel-coated plates with Essential 8 medium (GIBCO) and passaged with TrypLE TM express (GIBCO). 10 µM Peramivir (M3222, AbMole BioScience), 1 µM Nucleozin (A3670, Apexbio), 50 µM PYC-12, 20 µM RO3306, 10 µM WHL-50B, 20 ng/mL BDNF (450-02, PeproTech), 20 ng/mL GDNF (450-10, PeproTech), 20 ng/mL NT3 (450-03, PeproTech) were used in this study.
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