Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation and cryopreserved in liquid nitrogen until analysis. Immunophenotypic analysis was performed using the following directly conjugated anti-human antibodies: APC-Cy7 CD3 (Clone SP34-2), V500 CD4 (clone L200), PE-CD95 (clone DX2), PECy7-CD16 (clone 3G8), and PE-γδ TCR (clone B1) were obtained from BD Biosciences; APC-CCR7 (R&D; clone 150503); AF405-CD8 (Caltag; clone 3B5), PerCpCy5.5-CD28 (Biolegend; clone CD28.2), AF700-CD45RA (Abd Serotec; clone F8-11-13) and PECy7-CD127 (Biolegend; clone A019D5) were also used in these analysis. Cell proliferation was monitored by staining with AF700- or FITC-Ki-67 Ab in cells permeabilized with the Foxp3 Staining Buffer Set (eBioscience). All the samples were acquired in a LSR II Flow Cytometer (BD) and analyzed using FlowJo software (Tree Star, Inc., Ashland, OR).
Pe γδ tcr clone b1
Manufactured by BD
Sourced in United States
PE-γδ TCR (clone B1) is a laboratory reagent used for the detection of gamma-delta T cell receptor (TCR) expression on cells. It is a monoclonal antibody conjugated to the fluorescent dye phycoerythrin (PE), which allows for the identification and quantification of gamma-delta TCR-positive cells using flow cytometry.
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Lab products found in correlation
2 protocols using pe γδ tcr clone b1
1
Immunophenotypic Analysis of PBMCs
2
Multiparametric Immunophenotypic Analysis of PBMC
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Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation and cryopreserved in liquid nitrogen until analysis. Immunophenotypic analysis was performed using the following directly conjugated anti-human antibodies: APC-Cy7 CD3 (clone SP34-2), V500 CD4 (clone L200), PE-CD95 (clone DX2), PECy7-CD16 (clone 3G8) and PE-γδ TCR (clone B1) were obtained from BD Biosciences (San Diego, CA, USA); APC-CCR7 (R&D Systems; clone 150503); AF405-CD8 (Caltag, Buckingham, UK; clone 3B5), PerCpCy5.5-CD28 (Biolegend, San Diego, CA, USA; clone CD28.2), AF700-CD45RA (Abd Serotec, Raleigh, NC, USA; clone F8-11-13) and PECy7-CD127 (Biolegend; clone A019D5) were also used in these analysis. Cell proliferation was monitored by staining with AF700- or FITC-Ki-67 Ab in cells permeabilized with the Foxp3 Staining Buffer Set (eBioscience, San Diego, CA, USA). All the samples were acquired in a LSR II Flow Cytometer (BD Biosciences) and analyzed using FlowJo software (Tree Star, Inc., Ashland, OR, USA).
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