Rat anti mouse mhc class 2 1 a 1 e m5 114

Manufactured by Thermo Fisher Scientific

The Rat anti-mouse MHC Class II (I-A/I-E; M5/114.15.2) is a monoclonal antibody that can be used to detect the expression of mouse major histocompatibility complex (MHC) class II molecules, specifically the I-A and I-E isotypes. This antibody binds to a common epitope shared by the I-A and I-E molecules, allowing for the identification of cells expressing these MHC class II proteins.

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7 aad, by BioLegend (1 mentions)

Spelling variants of this product

M5/114.15.2 (19 citations) MHC class II (16 citations) MHC-class II (M5/114.15.2, (4 citations) Anti-MHC-I (4 citations) Anti-MHC class II (I-A/I-E) (M5/114.15.2, (2 citations) Anti–MHC class II (M5/114.15.2, (2 citations) Anti-MHC class I (2 citations) Anti-MHC class II (2 citations) MHC class I (2 citations) Anti-mouse MHC-II (2 citations)

1 protocol using rat anti mouse mhc class 2 1 a 1 e m5 114

Flow Cytometric Analysis of DCs and Splenic Cells

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After the culture for 10 days with GM-CSF and IL-4, the DCs were collected and stained for marker proteins on the cell surface using specific fluorophore-conjugated antibodies; Armenian hamster anti-mouse CD11c (N418), Armenian hamster anti-mouse CD80 (16-10A1), rat anti-mouse CD86 (GL-1), rat anti-mouse CD54/ICAM1 (YN1/1.7.4, all from BioLegend), and rat anti-mouse MHC Class II (I-A/I-E; M5/114.15.2, eBioscience). Splenic cells isolated from 30-week-old mice were stained with Armenian hamster anti-mouse CD11c (N418, BioLegend) and rat anti-mouse MHC Class II (I-A/I-E; M5/114.15.2, eBioscience). Dead cells were excluded by 7-AAD (BioLegend) staining. All samples were analyzed in a flow cytometer (FACSCanto II) and Flowjo software.

Kawahara K., Mukai T., Iseki M., Nagasu A., Nagasu H., Akagi T., Tsuji S., Hiramatsu-Asano S., Ueki Y., Ishihara K., Kashihara N, & Morita Y. (2021). SH3BP2 Deficiency Ameliorates Murine Systemic Lupus Erythematosus. International Journal of Molecular Sciences, 22(8), 4169.

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