Microview software v2

Eight bone samples from each noninfected group included in the aforementioned model were fixed in 10% formaldehyde for 48 h at 4 °C. After fixation, the samples were dehydrated in 96% ethanol for 48 h, changing the ethanol every 24 h, and in 100% ethanol for 48 h, changing the ethanol every 24 h. Hind legs were removed and fixed in 10% neutral buffered formalin. Before CT scanning, the paws were washed with running water for 15 min. Three-dimensional microcomputed tomographic imaging was performed with a CompaCT scanner (SEDECAL Madrid, Spain). Data were acquired with 720 projections by 360° scan, the integration time of 100 ms with three frames, a photon energy of 50 KeV, and current of 100 uA. The duration of imaging time was 20 min per scan. Three-dimensional renderings of images of hind paws were generated through original volumetric reconstructed images by MicroView software (GE Healthcare, Boston, MA, USA). Comparable regions of interest consisting of three metatarsal joints from each mouse were selected for analysis. Bone volume (BV), bone mass (BM), BMD (calculated as BM/BV mg/cm³), and mean cortical thickness (mm) were quantified from micro-CT scans using GE MicroView software v2.2.

Whole-body scans were collected in 6-week-, 11-week-, 12-month-, and 18-month-old control and Mig-6^over/over male and female mice. Mice were euthanized and imaged using the General Electric (GE) SpeCZT microCT machine [45 (link)] at a resolution of 50 μm/voxel or 100 μm/voxel. GE Healthcare MicroView software (v2.2) was used to generate 2D maximum intensity projection and 3D isosurface images to evaluate skeletal morphology. MicroView was used to create a line measurement tool in order to calculate the bone lengths; femur lengths were calculated from the proximal point of the greater trochanter to the base of the lateral femoral condyle. Tibia lengths were measured from the midpoint medial plateau to the medial malleolus. Humerus lengths were measured from the midpoint of the greater tubercle to the center of the olecranon fossa.