H1299

Cell lines and cell culture. The human NSCLC NCI-H1299 (H1299) cell was purchased from American Type Culture Collection (ATCC, Manassas, VA). H1299 cells were grown on Dulbecco's modified Eagle's minimum essential medium and Ham's F12 medium (D-MEM/Ham's F-12; WAKO, Tokyo, Japan) containing 10% fetal bovine serum (FBS; Phagokinetic track assay. A phagokinetic track assay was conducted according to the method of Alberecht-Buehler (1) . A gold solution was prepared as follows: Na 2 CO 3 and HAuCl 4 were mixed to give final concentrations of 10.6 and 1.27 mM, respectively, and heated on a gas burner; immediately after boiling, formaldehyde was added to give a final concentration of 0.01%. The gold solution was dropped onto a cover slip coated with 1% BSA, which was placed in a 35-mm culture dish and left to stand for 45 min. The slip was then washed twice with DMEM, following which DMEM containing 2% FBS was added. The cells (3 × 10 3 ) were plated onto the cover slip and cultured for 24 h in an incubator at 37°C and 5% CO 2 . They were then observed under an inverted microscope. Five random fields of view were photographed in each well at 40× magnification to measure the area of migration for each cell using Photoshop (Adobe Systems, San Jose, CA). The assay was conducted for three wells per sample.