Tapestation 2200 dna analyzer

The percentage of HMW DNA recovered (%R) was calculated using data obtained from the Agilent Technologies TapeStation 2200 DNA Analyzer as follows:
$\mathrm{\%}R=\frac{ng/\mu lHMWDNA(>10kb)}{ng/\mu ltotalDNA}\times 100\mathrm{\%}$
Normalized HMW DNA yield (nY) was calculated as follows using data obtained from the Agilent Technologies TapeStation 2200 DNA Analyzer and extract tissue weights modified by the correction ratio explained above:
$nY=\frac{\mu gHMWDNA(>10kb)}{mgextracttissueweight\times correctionratio}$
Data for each taxon, time interval and response variable (%R and nY) were analyzed separately. A Shapiro-Wilk normality test was used to determine whether data were normally distributed. For each taxon, the effect of storage solution on DNA quality was assessed using a repeated measures design: normally distributed data were analyzed using a parametric repeated measures ANOVA and non-normal data were analyzed using a non-parametric Friedman χ² test (RStudio v. 1.2.1335). Post-hoc tests for repeated measures ANOVAs were performed with the ‘nlme’ package in RStudio (Version 3.1–137). We used the Friedman Conover test as a post-hoc test for Friedman χ² analyses and performed them with a Bonferroni correction in the ‘PMCMR’ package (Version 4.3).

In our analyses, DNA fragments greater than 10 kb were considered HMW DNA. To estimate the quantity of HMW DNA, we first used the Agilent Technologies TapeStation 2200 DNA Analyzer (Santa Clara, CA) to evaluate the quantity of low molecular weight DNA (150 bp to 10 kb) and the total amount of DNA present in each sample. Then we calculated the quantity of HMW DNA for each sample by subtracting the low molecular weight DNA from the total amount of DNA present. Finally, we determined the percent of HMW DNA recovered (%R) using the following formula:
$$\%R=\frac{\frac{ng}{\mu l}HMWDNA(>10kb)}{\frac{ng}{\mu l}totalDNA}*100\%$$
Normalized HMW DNA yield (nY) was calculated as follows:
$$nY=\frac{\mu gHMWDNA(>10kb)}{mgextracttissueweight}$$
Data for each taxon were analyzed separately in RStudio version 1.4.1717 [29 , 30 ]. A Shapiro-Wilk normality test was used to determine if the data were normally distributed. If data were not normally distributed, a non-parametric Friedman χ² followed by a Bonferroni-corrected Friedman Conover post-hoc test was used to test the effect of storage solution on DNA quality using the “PMCMR” package version 4.3 [31 ]. If the data were normal, a one-way repeated measures ANOVA followed by a Bonferroni-corrected Tukey post-hoc test was performed with the “nlme” package [32 ].