Plan apo na 1.4 63 phase contrast oil immersion objective

Manufactured by Leica

The Plan apo NA/1.4 63× phase contrast oil immersion objective is a high-performance microscope objective designed for advanced microscopy applications. It features a numerical aperture of 1.4 and a magnification of 63×, providing high-resolution imaging capabilities. The objective is optimized for phase contrast microscopy techniques, which enhance the visibility of transparent specimens.

Automatically generated - may contain errors

Lab products found in correlation

Neo v5, by Oxford Instruments (2 mentions) Dm5500b, by Leica (2 mentions) Hoechst 33342, by Leica (1 mentions) Hoechst 33342, by Merck Group (1 mentions)

Close spelling variants of this product

63× oil-immersion objective (49 citations) Plan Apo (25 citations) 63×/1.4-NA oil-immersion objective (15 citations) Plan Apo 63× (14 citations) Plan Apo 63×/1.4 NA oil immersion objective (8 citations) Plan Apo objective (7 citations) 63x NA 1.4 oil objective (6 citations) 63 × 1.4 NA objective (6 citations) 1.4 NA 63× oil-immersion objective (3 citations) Plan Apo 63/1.4 NA oil (2 citations)

2 protocols using plan apo na 1.4 63 phase contrast oil immersion objective

Live-cell Fluorescence Microscopy Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Live-cells were stained with Hoechst 33342 (Sigma-Aldrich, B2261) and adhered, live, to glass slides as previously described (Dean and Sunter, 2020 (link)). mNG and Hoechst 33342 fluorescence and phase-contrast micrographs were captured on the same microscope and using identical settings as the TrypTag genome wide protein localisation project, a DM5500 B (Leica) upright widefield epifluorescence microscope using a plan apo NA/1.4 63× phase contrast oil immersion objective (Leica, 15506351) and a Neo v5.5 (Andor) sCMOS camera using MicroManager (Edelstein et al., 2010 ).

Jeilani M., Billington K., Sunter J.D., Dean S, & Wheeler R.J. (2022). Nucleolar targeting in an early-branching eukaryote suggests a general mechanism for ribosome protein sorting. Journal of Cell Science, 135(19), jcs259701.

+ Open protocol

+ Expand

Preparing Cells for Fluorescence Microscopy

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were prepared for light microscopy by centrifugation to remove medium, followed by resuspension in FCS-free HMI-9 containing 1 µg/mL Hoechst 33342 before a second centrifugation and resuspension in a small volume (~20 µl) of FCS-free HMI-9. An equal volume of 0.04% (v/v) formaldehyde in FCS free HMI-9 was added to lightly fix the cells
^{17 (link),
23 (link)}. Images were captured on a DM5500 B (Leica) upright widefield epifluorescence microscope using a plan apo NA/1.4 63× phase contrast oil-immersion objective (Leica, 15506351) and a Neo v5.5 (Andor) sCMOS camera using MicroManager (version 1.4.18)
^{24 (link)}.

Halliday C., Dean S., Sunter J.D, & Wheeler R.J. (2023). Subcellular protein localisation of Trypanosoma brucei bloodstream form-upregulated proteins maps stage-specific adaptations. Wellcome Open Research, 8, 46.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!