The whole sample preparation and data acquisition were done at CEITEC MU. The 3.5 μl of SDBC sample (5 mg/ml) was applied to freshly glow-discharged transmission electron microscopy grids (Protochips, Cu, 300 mesh, R1.2/1.3) and vitrified into liquid ethane using ThermoScientific Vitrobot Mark IV (4 °C, 100% rel. humidity, 30 s waiting time, 3 s blotting time). The grids were subsequently mounted into the Autogrid cartridges and loaded to a FEI Talos Arctica (ThermoScientific) transmission electron microscope for screening prior to data acquisition. The SDBC data were collected using a FEI Titan Krios operated at 300 kV using the SerialEM software (https://bio3d.colorado.edu/SerialEM/ ; 28 (link)). The microscope was aligned for fringe-free imaging. The data were collected on the K2 direct electron detection camera (Gatan) positioned behind the Gatan Imaging Filter at the calibrated pixel size of 0.818 Å/px (nominal magnification of 1,650,00×). The energy selecting slit was set to 10 eV. The data from a 5.0 s exposure were saved into 40 frames containing overall dose of 55 e/Å2. The dataset comprised 9594 movies in total. The image defocus was set to to vary between −0.5 μm and −1 μm.
K2 direct electron detection camera
Manufactured by Ametek
Sourced in United States
The K2 direct electron detection camera is a high-performance imaging device designed for use in electron microscopy applications. It captures direct electron images without the need for scintillators or other conversion layers, enabling high-resolution and low-noise imaging.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using k2 direct electron detection camera
1
Cryo-EM Sample Preparation and Data Acquisition
2
Characterizing Aluminum Impurity Deposits
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Sigma 300 VP
(Zeiss, Germany) scanning electron microscope equipped with an Oxford
SDD 30 EDS detector (Oxford Instruments, United Kingdom) was used.
Transmission electron microscopy (TEM) lamellae were prepared by an
FEI Nova 200 NanoLab (FEI, USA), a dual beam focused ion beam (FIB)/SEM
microscope, and mounted at an Omniprobe Cu grid. Measurements were
performed with an FEI Titan3 (link) G2 60-300
(FEI, USA) equipped with a CS-probe corrector, a Super-X EDS detector
(FEI, NL), and a Gatan Image Filter Quantum ERS (Gatan, USA) as well
as a K2 direct electron detection camera (Gatan, USA). All TEM measurements
were carried out at 300 kV acceleration voltage and in monochromated
STEM mode. Elemental maps and line scans were obtained using GMS 3
(Gatan, USA). The HAADF SE image of the impurity deposit and corresponding
TEM-EDS maps (Figure S6b–h ) were
acquired and processed using Velox Version 2 (Thermo Fischer Scientific,
USA). As for SNMS and XPS, it was necessary also for TEM to use thin
film samples because the surface of mechanically polished Al is too
rough.
(Zeiss, Germany) scanning electron microscope equipped with an Oxford
SDD 30 EDS detector (Oxford Instruments, United Kingdom) was used.
Transmission electron microscopy (TEM) lamellae were prepared by an
FEI Nova 200 NanoLab (FEI, USA), a dual beam focused ion beam (FIB)/SEM
microscope, and mounted at an Omniprobe Cu grid. Measurements were
performed with an FEI Titan3 (link) G2 60-300
(FEI, USA) equipped with a CS-probe corrector, a Super-X EDS detector
(FEI, NL), and a Gatan Image Filter Quantum ERS (Gatan, USA) as well
as a K2 direct electron detection camera (Gatan, USA). All TEM measurements
were carried out at 300 kV acceleration voltage and in monochromated
STEM mode. Elemental maps and line scans were obtained using GMS 3
(Gatan, USA). The HAADF SE image of the impurity deposit and corresponding
TEM-EDS maps (
acquired and processed using Velox Version 2 (Thermo Fischer Scientific,
USA). As for SNMS and XPS, it was necessary also for TEM to use thin
film samples because the surface of mechanically polished Al is too
rough.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!