Total erk

Manufactured by Merck Group

Sourced in United States

Total ERK is a laboratory equipment product that measures the total amount of extracellular signal-regulated kinase (ERK) protein present in a sample. ERK is a critical signaling molecule involved in various cellular processes. The Total ERK product provides a quantitative assessment of the total ERK levels in a given biological sample.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Anti-total ERK (2 citations)

4 protocols using total erk

Immunoblotting Analysis of Neural Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblotting was carried out as previously reported.^{4 (link)} Brains, optic nerves and cultured oligodendrocytes were freshly isolated, and then homogenized. Samples were separated on a sodium dodecyl sulfate-PAGE and subsequently transferred to an Immobilon-P filter (Millipore, Billerica, MA, USA). Membranes were incubated with an antibody against Dock3 (1 : 1000), Neu-N (1 : 1000, Millipore), NG2 (1 : 500, Millipore), GFAP (1 : 500, Santa Cruz), CNPase (1 : 1000, Sigma), Elmo, total Erk, phosphorylated Erk, total p38, phosphorylated p38, total JNK, phosphorylated JNK or actin (1 : 1000; BD Biosciences).

Namekata K., Kimura A., Harada C., Yoshida H., Matsumoto Y, & Harada T. (2014). Dock3 protects myelin in the cuprizone model for demyelination. Cell Death & Disease, 5(8), e1395-.

+ Open protocol

+ Expand

Investigating Platelet Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gel-purified platelets were incubated with 10 nmol/L 12(S)-HETE in the presence of 1 and 3 μM GPR310 or with 30 μM SFLLRN in a platelet aggregometer at 37 °C for various times. Platelets were solubilized in SDS-PAGE sample buffer.^{12 (link)} In other experiments, gel-purified platelets were incubated with 3 nmol/L thrombin in the presence or absence of 1 μM GPR310. Equal amounts of protein were resolved by 10% SDS-PAGE gel for Western analysis. The primary antibodies used were human phospho-p38 (Thr180/Tyr182; Cell Signaling; No. 4631), total p38 (Cell Signaling; No. 9212), phospho-Akt (Ser473; Cell Signaling; No. 4058S), total-Akt (No. 9272), phospho-Erk (Thr202/Tyr204; Cell Signaling; No. 4370), total-Erk (No. 9102S), and actin (Sigma Aldrich; No. A1978).

Van Doren L., Nguyen N., Garzia C., Fletcher E.K., Stevenson R., Jaramillo D., Kuliopulos A, & Covic L. (2020). Lipid Receptor GPR31 (G-Protein–Coupled Receptor 31) Regulates Platelet Reactivity and Thrombosis Without Affecting Hemostasis. Arteriosclerosis, thrombosis, and vascular biology, 41(1), e33-e45.

+ Open protocol

+ Expand

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

ASM cells were lysed and the proteins (10–12 µg) were resolved on a 10–12% SDS-PAGE, then transferred onto nitrocellulose membranes (Bio-Rad, USA) as previously described³¹. Antibodies used were sm-α-actin (1:3000), β-actin (1:15000), α2-chain laminin (1:500), Hras (1:200), Kras (1:300), Nras (1:500) (all from Sigma, USA); phospho-ERK, total ERK, phospho-p38 MAPK, total p38 MAPK, p21 Ras, phospho-PKCα, total PKCα, phospho-PKCς, total PKCς, phospho-p70^S6K, total p70^S6K, cyclin D1 (all 1:1000) (all from Cell Signaling, USA); and integrin α7 (1:500) (Abcam, Cambridge, UK). Proteins were visualized on Kodak film after incubation with enhanced chemiluminescence reagents, then exposure levels were quantified by TotalLab (UK) densitometry software. Results were expressed as fold increment over Day 0 relative to β-actin.

Teoh C.M., Tan S.S., Langenbach S.Y., Wong A.H., Cheong D.H., Tam J.K., New C.S, & Tran T. (2019). Integrin α7 expression is increased in asthmatic patients and its inhibition reduces Kras protein abundance in airway smooth muscle cells. Scientific Reports, 9, 9892.

+ Open protocol

+ Expand

Immunoblotting and Immunohistochemistry of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The level of protein expression in cultured cells was determined by immunoblotting as previously described [17 (link),18 (link)] using primary antibodies against MMP-9 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), p53, phospho-ERK1/2, total ERK, and β-actin (all Sigma-Aldrich, St. Louis, MO, USA). Protein expression in xenografted tumors was analyzed by immunohistochemistry. Tumor tissues were fixed immediately after harvesting in 10% phosphate-buffered formalin, embedded in paraffin, and tissue sections were stained with the antibodies indicated above.

Liu C., Dai L., Liu Y., Rong L., Dou D., Sun Y, & Ma L. (2016). Antiproliferative Activity of Triterpene Glycoside Nutrient from Monk Fruit in Colorectal Cancer and Throat Cancer. Nutrients, 8(6), 360.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!