Two human cSCC cell lines, HSC-1 and A431, were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) and the Korean Cell Line Bank (Seoul, Korea), respectively, for use in this study. Cell lines were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco Biosciences, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen, Waltham, MA, USA). pGFP-C-shMAGEA12 lentiviral particle (OriGene, Rockville, MD, USA) was used to establish HSC-1 and A431 cells with stable MAGEA12 knockdown (HSC-1-MAGEA12 Δ and A431-MAGEA12 Δ , respectively). Silencing of MAGEA12 was confirmed with immunocytochemistry. Lentiviral particles for pGFP-C-shLenti vector weve used to establish control stable cell lines (HSC-1-Mock and A431-Mock).
Hsc 1
Manufactured by OriGene
Sourced in United States
The HSC-1 is a laboratory equipment designed for high-speed cell sorting. It is capable of rapidly and accurately separating different cell populations based on specific fluorescent markers or other physical characteristics. The core function of the HSC-1 is to enable efficient cell sorting for various research and diagnostic applications.
Automatically generated - may contain errors
2 protocols using hsc 1
1
Establishment of MAGEA12 Knockdown cSCC Cell Lines
2
Establishment of Stable Cell Lines for cSCC Research
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Two human cSCC cell lines, A431 and HSC-1, were purchased from the Korean Cell Line Bank (Seoul, South Korea) and the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan), respectively, for in vitro and in vivo analyses. COPB2 knockdown stable HSC-1 and A431, as well as related control cells, were established using pGFP-C-shCOPB2 lentiviral particles (OriGene, Rockville, MD, USA) and maintained in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% fetal bovine serum (FBS; Invitrogen, Waltham, MA, USA) and 1% streptomycin/penicillin (Gibco, Waltham, MA, USA).
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